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Anti iab fitc

Manufactured by BioLegend
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Anti-IAb-FITC is a fluorescently-labeled antibody that binds to the IAb molecule, a major histocompatibility complex (MHC) class II antigen expressed on antigen-presenting cells in mice. This product can be used for the identification and analysis of IAb-expressing cells by flow cytometry.

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Lab products found in correlation

Anti b220 apc cy7, by BioLegend (2 mentions) Anti cd4 fitc, by BioLegend (1 mentions) Anti cd4 pe, by BioLegend (1 mentions) Anti cd117 pe cy7, by BioLegend (1 mentions) Anti cd8 apc, by BioLegend (1 mentions) Anti cd34 pe, by BioLegend (1 mentions) Anti cd93, by BioLegend (1 mentions) Anti igm pe, by BioLegend (1 mentions) Anti cd11c, by BioLegend (1 mentions) Anti cd11b fitc, by Thermo Fisher Scientific (1 mentions) Anti f4 80 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd4 fitc, by BD (1 mentions) Anti cd8 apc cy7, by BD (1 mentions) Anti cd11c fitc, by Thermo Fisher Scientific (1 mentions) Anti b220 fitc, by BD (1 mentions) Anti cd44 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti cd3 percp cy5, by BD (1 mentions) Anti cd11c percp cy5, by BioLegend (1 mentions)

3 protocols using anti iab fitc

1

Comprehensive Immunophenotyping Panel

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The following antibodies were purchased from Biolegend Inc. (San Diego, CA, USA): anti-CD3-pacific blue (17A2), anti-CD4-pacific blue (GK1.5), anti-NK1.1-pacific blue (PK136), anti-CD11b-pacific blue (M1/70), anti-CD11C-pacific blue (N418), anti-Ter-119-pacific blue (Ter-119), anti-CD8-pacific blue (53-6.7), anti-B220-pacific blue (RA3-61B2), anti-CD4-PE (GK1.5), anti-CD4-FITC (GK1.5), anti-CD8-APC (53-6.7), anti-CD8-Pecy7 (53-6.7), anti-CD23-PECy7 (B3B4), anti-CD21/CD35 (CR2/CR)-PerCP/Cy5.5 (7E9), anti-Gr1-Pecy7 (RB6-8C5), Anti-IAb-FITC (KH74), anti-B220-APC-Cy7 (RA3-61B2), anti-CD117-PE-Cy7 (2B8), anti-PDCA1-APC (927), anti-CD135 (flt3)-APC (A2F10), anti-CD25-PE (3C7), anti-CD11C (N418), anti-IgD-PerCP/Cy5.5 (11-26c.2a), anti-Sca1-APC (D7), anti-IgM-PE (RMM-1), anti-CD16/32-APC/Cy7(93), anti-CD127 (IL-7R)-PerCP/Cy5.5 (SB/199), anti-CD93 (AA4.1)-PE (AA4.1), anti-CD117 (c-kit)-PE/Cy7 (2B8), and anti-CD34-PE (MEC14.7). Anti-human/mouse phospho-ERK1/2 (T202/Y204)-APC (MILAN8R), anti-human-mouse phospho-p38 (T180/Y182)-APC (4NIT4KK), and anti-human/mouse phosphor-AKT (S473)-APC (SDRNR) were purchased from eBioscience (San Diego, CA, USA).
Yang Y., Xu J., Chen H., Fei X., Tang Y., Yan Y., Zhang H, & Zhang J. (2016). MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget, 7(14), 17520-17531.
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2

Characterization of WT1-Specific T Cells

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TAME was performed as previously described31 (link), 32 (link) in HLA-A2.1 transgenic mice. Briefly, single-cell suspensions of pooled spleen and lymph nodes were stained with WT1A-PE, WT1B-APC or both tetramers before magnetic enrichment. Mice were randomly divided into groups of five. Enriched cells were then stained with mouse anti-CD3 PerCP-Cy5.5, anti-CD8 APC-Cy7 (#557654, BD Biosciences), anti-CD44 PE-Cy7 (#25044182, eBiosciences), anti-CD62L BV570 (#104433, Biolegend), anti-CD4 FITC (#553055, BD Biosciences), anti-B220 FITC (#553088, BD Biosciences), anti-F4/80 FITC (#11480185, eBiosciences), anti-CD11b FITC (#11011282, eBiosciences), anti-CD11c FITC (#11011482, eBiosciences), anti-I-Ab FITC (#116406, Biolegend) and LIVE/DEAD Fixable Aqua before acquiring using the LSR Fortessa II.
Nguyen T.H., Tan A.C., Xiang S.D., Goubier A., Harland K.L., Clemens E.B., Plebanski M, & Kedzierska K. (2017). Understanding CD8+ T-cell responses toward the native and alternate HLA-A*02:01-restricted WT1 epitope. Clinical & Translational Immunology, 6(3), e134-.
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3

Antibody Panel for Immune Cell Analysis

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The following antibodies were purchased from Biolegend Inc.: anti-CD3-pacific blue (17A2), anti-CD4-pacific blue (GK1.5), anti-CD4-FITC (GK1.5), anti-CD8-pacific blue (53-6.7), anti-CD8-APC (53-6.7), anti-NK1.1-pacific blue (PK136), anti-CD11b-pacific blue (M1/70), anti-CD11c-pacific blue (N418), anti-CD11c-percp-cy5.5 (N418), anti-I-Ab-FITC (KH74), anti-I-Ab-PE (KH74), anti-CD34-PE (MEC14.7), anti-B220-APC-Cy7 (RA3-61B2), anti-B220-pacific blue (RA3-61B2), anti-CD117-PE-Cy7 (2B8), anti-PDCA1-APC (927), anti-CD135-APC (A2F10), anti-CD172-biotin (P84), anti-IL-17a-PE (TC11-18H10.1), and strepedavidin-PE-Cy7 (Biolegend, CA). The anti-WNT1 antibody was purchased from affinity Bioscience (Affinity, OH).
Huang A., Yang Y., Chen S., Xia F., Sun D., Fang D., Xiong S., Jin L, & Zhang J. (2017). MiR-34a promotes DCs development and inhibits their function on T cell activation by targeting WNT1. Oncotarget, 8(10), 17191-17201.
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