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Cryptotanshinone

Manufactured by Yuanye Bio-Technology
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Sourced in China

Cryptotanshinone is a bioactive compound extracted from the root of Salvia miltiorrhiza, a traditional Chinese medicinal herb. It serves as a versatile laboratory reagent for scientific research. Cryptotanshinone's primary function is to facilitate the study of various biological and biochemical processes in controlled laboratory settings.

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Lab products found in correlation

Rosmarinic acid, by Yuanye Bio-Technology (4 mentions) Tanshinone iia, by Yuanye Bio-Technology (3 mentions) Formononetin, by Yuanye Bio-Technology (2 mentions) Calycosin 7 o β d glucoside, by Yuanye Bio-Technology (2 mentions) Salvianolic acid b, by Yuanye Bio-Technology (2 mentions) P eif4e, by Abcam (1 mentions) P src, by Cell Signaling Technology (1 mentions) Dasatinib, by Yuanye Bio-Technology (1 mentions) P bcr abl, by Cell Signaling Technology (1 mentions) Pb9026, by Boster Bio (1 mentions) Eif4e, by BD (1 mentions) Bcr abl, by Cell Signaling Technology (1 mentions) P stat3, by Cell Signaling Technology (1 mentions) Bm0104, by Boster Bio (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Water of lc ms grade, by Thermo Fisher Scientific (1 mentions)

5 protocols using cryptotanshinone

1

Evaluation of Cryptotanshinone in Leukemia

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Cryptotanshinone (CPT) was purchased from Shanghai Yuanye Biotechnology Co., Ltd, with a purity of 98% (#B21586, Shanghai, China). High-performance liquid chromatography (HPLC) analysis was applied to further confirm the purity of CPT (98%). Imatinib (Genike) was obtained from Nanjing Chia Tai Tianqing Pharmaceutical Co., Ltd. The second-generation tyrosine kinase inhibitors nilotinib was purchased from Aladdin Chemistry Co., Ltd (#N126111, Shanghai, China). Dasatinib was obtained from Shanghai Yuanye Biotechnology Co., Ltd (#S45672, Shanghai, China). Antibodies used in this study were purchased from Cell Signalling Technology (MA, USA), including cleaved caspase-3 (#9664), cleaved PARP (#5625), p-STAT3 (#9145), Bcr-Abl (#3908), p-Bcr-Abl (#3009), p-Src (#2101) and β-Actin (#3700). Antibody for cleaved caspase-9 (Asp353) (#AF5240) was obtained from Affinity Biosciences (Cincinnati, OH, USA), for eIF4E (#610270) was obtained from BD Biosciences (San Diego, CA, USA), and p-eIF4E (ab76256) was purchased from Abcam. The rabbit polyclonal antibody against Ki-67 (PB9026) and mouse monoclonal antibody against PCNA (BM0104) was obtained from Boster Biological Technology Co., Ltd.
Cheng R., Huang Y., Fang Y., Wang Q., Yan M, & Ge Y. (2021). Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia. Pharmaceutical Biology, 59(1), 891-901.
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2

Standardized Analysis of Traditional Chinese Medicine

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Twenty-four authentic standards of adenosine (12), caffeic acid (17b), danshensu (17c), protocatechuic aldehyde (21), trans-5-caffeoylquinic acid (23b), ferulic acid (30), lithospermic acid (45b), salvianolic acid B (48a), baicalin (50a), ononin (50b), salvianolic acid A (61a), salvianolic acid C (70), astragaloside IV (77b), astragaloside III (79b), astragaloside II (83d), farrerol (84b), isoastragaloside II (85b), wogonin (86a), astragaloside I (92b), cyclosieversigenin (97b), dihydrotanshinone I (102a), tanshinone I (112), levistolide A (121) and tanshinone IIA (123) were all purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Five reference standards of calycosin7-O-β-d-glucoside (28b), rosmarinic acid (44), calycosin (62a), formononetin (80) and cryptotanshinone (130) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The structures of these reference standards (purity > 98%) are shown in Fig. 2. They were stored at 4 °C before use.
Acetonitrile, formic acid and water of LC-MS grade were purchased from Fisher Scientific Company (USA). Methanol of analytical grade was obtained from Runjie Tech Co. (Shanghai, China).
DZT and its five ingredients, i.e., LC, SM, PA, AM, and HN were provided by Tonghua Baishan Pharmaceutical Co., Ltd. (Jilin, China).
Cheng T., Ye J., Li H., Dong H., Xie N., Mi N., Zhang Z., Zou J., Jin H, & Zhang W. (2019). Hybrid multidimensional data acquisition and data processing strategy for comprehensive characterization of known, unknown and isomeric compounds from the compound Dan Zhi Tablet by UPLC-TWIMS-QTOFMS. RSC Advances, 9(16), 8714-8727.
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3

HPLC Analysis of Medicinal Compounds in Salvia miltiorrhiza

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HPLC-grade acetonitrile was purchased from Merck Co., Inc. (Darmstadt, Germany). HPLC-grade phosphoric acid and analytical-grade methanol were purchased from Tianjin Komiou Chemical Reagent Co., Ltd. (Tianjin, China). Six reference compounds (rosmarinic acid, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA, HPLC ≥ 98%) were purchased from Shanghai Yuan ye Bio-Technology Co., Ltd. (Shanghai, China). Ultrapure water was generated with a You pu Ultrapure Water System (Chengdu, China).
The fresh roots and rhizomes of one-year-old S. miltiorrhiza plants were harvested in 2020 in Tongchuan (Tongchuan, China) in April, the best time of year for the harvest of the medicinal parts of S. miltiorrhiza, and were identified by Professor Zongsuo Liang (Zhejiang Sci-tech University). After removing soil and impurities, the samples were divided into 16 batches according to their weight, size and thickness.
Zhang L., Zhang X., Begum N., Xia P., Liu J, & Liang Z. (2022). Effects of Different Processing Methods Based on Different Drying Conditions on the Active Ingredients of Salvia miltiorrhiza Bunge. Molecules, 27(15), 4860.
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4

Quantification of Bioactive Compounds

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Chemical standards, including danshensu, caffeic acid, rosmarinic acid, cryptotanshinone, sugiol, carnosic acid, tanshinone IIA, and miltirone, were purchased from Shanghai Yuanye Biotechnology Corporation Ltd. (Shanghai, China). The other chemicals and reagents were purchased as follows: acetonitrile and methanol (HPLC grade), Merck (Darmstadt, Germany); warfarin, Sigma-Aldrich (Madrid, Spain); chloroform, Sinopharm Chemical Reagent (Shanghai, China); leucine encephalin, Waters (Milfor, Massachusetts, USA); pure distilled water, Watsons water (Hong Kong, China); and formic acid (HPLC grade), Fisher Scientific (Fair Lawn, New Jersey, USA).
Hu J., Wang F., Liang F., Wu Z., Jiang R., Li J., Chen J., Qiu S., Wang J., Zhang Y., Li Q, & Chen W. (2022). Identification of Abietane-Type Diterpenoids and Phenolic Acids Biosynthesis Genes in Salvia apiana Jepson Through Full-Length Transcriptomic and Metabolomic Profiling. Frontiers in Plant Science, 13, 919025.
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5

Comprehensive Chemical Profiling of YGYSG

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The production of YGYSG was undertaken in The First Clinical Medical College at Anhui University of Chinese Medicine. A Milli-Q water purification system (Millipore, Bedford, MA, USA) was employed to acquire deionized water. For mass spectrometry analysis, formic acid of LC-MS grade was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile and methanol were LC-MS grade and obtained from Merck (Darmstadt, Germany). Overall, 27 chemical standards, including chikusetsusaponin IVa, chikusetsusaponin IV, ginsenoside Ro, 25R-inokosterone, β-ecdysone, acacetin, cymaroside, quercetin, gallic acid, oleanolic acid, hyperoside, chlorogenic acid, astragalin, kaempferol, cryptotanshinone, tanshinone IIA, rosmarinic acid, calycosin-7-O-β-D-glucoside, salvianolic acid B, astragaloside IV, formononetin-7-O-β-D-glycoside, calycosin, formononetin, scopoletin, ferulic acid, rutin, and narcissoside, were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), and their purity levels exceeded 98% as determined through high-performance liquid chromatography (HPLC) analysis. Figure 2 displays the chemical configurations of each reference standard.
Yang F., Zhang K., Dai X, & Jiang W. (2024). Preliminary Exploration of Potential Active Ingredients and Molecular Mechanisms of Yanggan Yishui Granules for Treating Hypertensive Nephropathy Using UPLC-Q-TOF/MS Coupled with Network Pharmacology and Molecular Docking Strategy. Journal of Analytical Methods in Chemistry, 2024, 7967999.
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