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Anti cd45.2 apccy7

Manufactured by BioLegend
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Anti-CD45.2-APCcy7 is a fluorochrome-conjugated antibody that binds to the CD45.2 antigen. CD45.2 is a protein tyrosine phosphatase expressed on the surface of hematopoietic cells. This antibody can be used to identify and differentiate cells expressing the CD45.2 marker.

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Lab products found in correlation

Anti f4 80 pecy7, by BioLegend (3 mentions) Anti cd8 pe cy7, by BioLegend (2 mentions) Anti gr 1 fitc, by BioLegend (2 mentions) Anti pd l1 pe, by BioLegend (2 mentions) Anti b220 apc cy7, by BioLegend (2 mentions) Pe anti rabbit igg, by BioLegend (2 mentions) Anti cd16 cd32, by BioLegend (2 mentions) Anti cd45.2 apc, by BioLegend (2 mentions) Anti cd11b pacif blue, by BioLegend (2 mentions) Anti h 2kb bound to siinfekl apc, by BioLegend (2 mentions) Anti mhcii bv605, by BioLegend (2 mentions) Anti cd11c fitc, by BioLegend (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Anti cd86 pe, by BioLegend (2 mentions) Anti f4 80 bv785, by BioLegend (1 mentions) Trustain fcx anti mouse cd16 32, by BioLegend (1 mentions) Anti nk1.1 fitc, by BioLegend (1 mentions) Anti cd3 bv421, by BioLegend (1 mentions) Anti cd19 pe cy5, by BioLegend (1 mentions) Anti cd4 buv563, by BD (1 mentions)

Close spelling variants of this product

Anti-APCCY7-CD45 Anti-CD45

3 protocols using anti cd45.2 apccy7

1

Multiparameter Flow Cytometry Analysis

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Antibodies were purchased from Biolegend. The following primary antibodies were used: anti-CD16/CD32 (Biolegend, Cat#: 101301, clone: 93), anti-CD8-PE-cy7 (Biolegend, Cat#: 100721, clone: 53-6.7), anti-CD11c-FITC (Biolegend, Cat#: 117305, clone: N418) and anti-CD11b-Pacif blue (Biolegend, Cat#: 101223, clone: M1/70), anti-B220-APC-cy7 (Biolegend, Cat#: 103223, clone: RA3-6B2), anti-CD86-PE (Biolegend, Cat#: 105007, clone: GL-1), anti- H-2Kb bound to SIINFEKL-APC (Biolegend, Cat#:141605, clone:25-D1.16), anti-CD45.2-APC (Biolegend, Cat#:109814, clone:104), anti-CD45.2-APCcy7 (Biolegend, Cat#:109823, clone:104), anti-PD-L1-PE (Biolegend, Cat#:124307, clone:10F.9G2), isotype control-PE (Biolegend, Cat#:400607, clone:RTK4530), anti-F4/80-PE/cy7 (Biolegend, Cat#:123113, clone:BM8), anti-Gr-1-FITC (Biolegend, Cat#:108419, clone:RB608C5), anti-MHCII-BV605 (Biolegend, Cat#:107639, clone:M5/114.15.2), anti-rabbit IgG-PE (Biolegend, Cat#:406421, clone:poly4064). Flow data were acquired on a BD™ LSR II flow cytometer and analyzed using Flowjo software.
Luo M., Wang H., Wang Z., Cai H., Lu Z., Li Y., Du M., Huang G., Wang C., Chen X., Porembka M.R., Lea J., Frankel A.E., Fu Y.X., Chen Z.J, & Gao J. (2017). A STING-Activating Nanovaccine for Cancer Immunotherapy. Nature nanotechnology, 12(7), 648-654.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were purchased from Biolegend. The following primary antibodies were used: anti-CD16/CD32 (Biolegend, Cat#: 101301, clone: 93), anti-CD8-PE-cy7 (Biolegend, Cat#: 100721, clone: 53-6.7), anti-CD11c-FITC (Biolegend, Cat#: 117305, clone: N418) and anti-CD11b-Pacif blue (Biolegend, Cat#: 101223, clone: M1/70), anti-B220-APC-cy7 (Biolegend, Cat#: 103223, clone: RA3-6B2), anti-CD86-PE (Biolegend, Cat#: 105007, clone: GL-1), anti- H-2Kb bound to SIINFEKL-APC (Biolegend, Cat#:141605, clone:25-D1.16), anti-CD45.2-APC (Biolegend, Cat#:109814, clone:104), anti-CD45.2-APCcy7 (Biolegend, Cat#:109823, clone:104), anti-PD-L1-PE (Biolegend, Cat#:124307, clone:10F.9G2), isotype control-PE (Biolegend, Cat#:400607, clone:RTK4530), anti-F4/80-PE/cy7 (Biolegend, Cat#:123113, clone:BM8), anti-Gr-1-FITC (Biolegend, Cat#:108419, clone:RB608C5), anti-MHCII-BV605 (Biolegend, Cat#:107639, clone:M5/114.15.2), anti-rabbit IgG-PE (Biolegend, Cat#:406421, clone:poly4064). Flow data were acquired on a BD™ LSR II flow cytometer and analyzed using Flowjo software.
Luo M., Wang H., Wang Z., Cai H., Lu Z., Li Y., Du M., Huang G., Wang C., Chen X., Porembka M.R., Lea J., Frankel A.E., Fu Y.X., Chen Z.J, & Gao J. (2017). A STING-Activating Nanovaccine for Cancer Immunotherapy. Nature nanotechnology, 12(7), 648-654.
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3

Multiparametric Immune Cell Analysis

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After incubating with FcBlock (TruStain FcX anti‐mouse CD16/32, Biolegend) to block non‐specific binding, the isolated non‐parenchymal liver cells and peripheral blood leukocytes were stained with fluorochrome‐conjugated monoclonal antibodies at 4 °C for 30 min in the dark and then washed with PBS. Cells were fixed and permeabilized before the staining of TLR9. Monoclonal antibodies included anti‐CD45.2 APC/Cy7 (Biolegend), anti‐CD11b BV510 (BD), anti‐F4/80 PE/Cy7 (Biolegend), anti‐F4/80 BV785 (Biolegend), anti‐Gr‐1 PE‐CF594 (BD), anti‐NK1.1 FITC (Biolegend), anti‐CD3 BV421 (Biolegend), anti‐CD4 BUV563 (BD), anti‐CD8a BV711 (Biolegend), anti‐CD19 PE/Cy5 (Biolegend), anti‐I‐A/I‐E Alexa Fluor 700 (Biolegend), and anti‐CD289 (TLR9) FITC (eBioscience). Data were collected using a flow cytometer (LSRFortessa or FACS Aria, BD) and analyzed using FlowJo V10 software.
Sun M., Chen P., Xiao K., Zhu X., Zhao Z., Guo C., He X., Shi T., Zhong Q., Jia Y., Tao Y., Li M., Leong K.W, & Shao D. (2023). Circulating Cell‐Free DNAs as a Biomarker and Therapeutic Target for Acetaminophen‐Induced Liver Injury. Advanced Science, 10(16), 2206789.
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