GH4C1 cells, a rat pituitary somatolactotroph line, were obtained in 2012 from ATCC® (CCL-82.2™, lot number: 58945448, Molsheim, France) with certificate analysis and were confirmed to be free of mycoplasma (MycoAlert, Lonza, Levallois-Perret, France). They were grown in HamF10 medium supplemented with 15% horse serum and 2% fetal calf serum.
Ccl 82.2
Manufactured by American Type Culture Collection
Sourced in France
CCL-82.2™ is a cell line derived from human colorectal adenocarcinoma tissue. It is intended for use in cell culture and biological research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ccl 82.2
1
Culturing Rat Pituitary Somatolactotroph Cells
2
Cultivation of GH4C1 Pituitary Cells
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GH4C1 cells, a rat pituitary somatolactotroph line, were obtained in 2012 from ATCC ® (CCL-82.2 ™ , lot number: 58945448, Molsheim, France) with certificate analysis and were confirmed to be free of mycoplasma (MycoAlert, Lonza, Levallois-Perret, France). They were grown in HamF10 medium supplemented with 15% horse serum and 2% fetal calf serum.
3
GH4C1 Cell Culture Protocol
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GH4C1 cells, a rat pituitary somatolactotroph line, were obtained from ATCC (CCL-82.2, lot number: 58945448) with certificate analysis and were confirmed to be free of mycoplasma (MycoAlert). They were grown in 10 cm cell dishes, in an incubator at 37°C, saturated with H 2 O and with 5% CO 2 . The HamF10 cell medium was supplemented with 15% horse serum, 2% fetal calf serum, 0.5% streptomycin and 0.5% penicillin. To synchronize cells between themselves and to be able to select the time of maximum Neat1 expression (12) , GH4C1 cells were transferred to fresh medium 24 to 30 h before crosslinking.
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