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Cd8a apc 53 6.7

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CD8a-APC (53-6.7) is a fluorescently-labeled monoclonal antibody that binds to the CD8a surface antigen. CD8a is a glycoprotein expressed on the surface of certain T cells and natural killer cells. The APC fluorescent label allows for detection and analysis of CD8a-positive cells using flow cytometry.

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Lab products found in correlation

Facscalibur cytometer, by BD (1 mentions) Digital lsr 2, by BD (1 mentions) Cd45.2 alexa700, by BioLegend (1 mentions) Anti mouse cd16 cd32 fc block, by BD (1 mentions)

Close spelling variants of this product

CD8a (53-6.7, (18 citations) CD8a-APC (10 citations) APC-Rat-Anti-Mouse-CD8a-(53-6.7) (2 citations)

2 protocols using cd8a apc 53 6.7

1

Multicolor Flow Cytometry Analysis

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Total splenocytes and CD4-depleted cell fractions were stained on ice for 20 min with the following antibodies: CD3e-PE (145-2C11; eBioscience), CD8a-APC (53-6.7; BD Pharmingen), CD4-FITC (H129.19; BD Pharmingen), and B220-PerCP-Cy5.5 (RA3-6B2; BD Pharmingen). The samples were run on a FACSCalibur cytometer (BD Bioscience), and data were analyzed with FlowJo software version 10 (TreeStar).
Soldemo M., Àdori M., Stark J.M., Feng Y., Tran K., Wilson R., Yang L., Guenaga J., Wyatt R.T, & Karlsson Hedestam G.B. (2017). Glutaraldehyde Cross-linking of HIV-1 Env Trimers Skews the Antibody Subclass Response in Mice. Frontiers in Immunology, 8, 1654.
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2

Phenotyping of Mouse Immune Cells

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PBMCs were blocked with 3.3 μg/ml anti-mouse CD16/CD32 (Fc Block; BD) in PBS containing 1% FBS and 0.1% sodium azide for 10 min on ice. Cells were then surface stained with cocktails of the following antibodies: Thy1.1 FITC (53-6.7; BD Biosciences), CD8 Pacific Blue (5H10; Life Technologies), CD8a APC (53-6.7; BD Biosciences), and CD45.2 Alexa700 (104; Biolegend) for 20 min on ice. All cells were acquired using a digital flow cytometer (Digital LSR II; BD), and data were analyzed with FlowJo software (Tree Star).
Gossa S., Nayak D., Zinselmeyer B.H, & McGavern D.B. (2014). Development of an Immunologically Tolerated Combination of Fluorescent Proteins for In vivo Two-photon Imaging. Scientific Reports, 4, 6664.
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