Sod cat no a001 1

An ultraviolet spectrophotometer was used to perform a colorimetric determination at a wavelength of 550 nm. Lipid peroxidation was determined by measuring the rate of production of thiobarbituric acid reactive substances (expressed as MDA equivalents) Lung tissues were added to precooled PBS (4°C) and then centrifuged (1,006.2 × g at 4°C for 15 sec) into tissue homogenates by a low-speed homogenate slurry machine (30 sec per time, interval ice bath for 1 min). Following centrifugation by the aforementioned methods, the tissue homogenates were first placed under a 37°C water bath for 40 min and then placed at room temperature for 10 min. The activity of SOD and the concentration of MDA were determined by a 550 nm colorimetric assay according to the manufacturer's protocol (SOD cat no: A001-1 and MDA cat no: A003-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Shenfu injection (Z51021920; 100 ml/ampulla; batch no. 100602) was purchased from Ya'an San-Jiu Pharmaceutical Co., Ltd. (Ya'an, China). TNF-α enzyme-linked immunosorbent assay (ELISA; cat no. MTA00B) MDA kits (cat no. A003-1) were provided by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. (Beijing, China). MPO (cat no. A044) and SOD (cat no. A001-1) kits were produced by the Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China).