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Eksigent nanolc 1d plus nanolc system

Manufactured by AB Sciex
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The Eksigent® NanoLC-1D™ Plus nanoLC™ system is a compact and robust liquid chromatography instrument designed for high-performance nanoflow separations. It features a high-precision nano-flow pump, an integrated column oven, and a sample injection system. The system is capable of delivering flow rates from 20 nL/min to 2 μL/min, making it suitable for a wide range of nanoLC applications.

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Lab products found in correlation

Thermo q exactive orbitrap mass spectrometer, by Thermo Fisher Scientific (5 mentions) Mascot, by Matrix Science (5 mentions)

Close spelling variants of this product

Eksigent nanoLC-1D plus system NanoLC-1D plus system Eksigent nanoLC system Eksigent nanoLC NanoLC 1D NanoLC system

5 protocols using eksigent nanolc 1d plus nanolc system

1

Protein Identification via Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

All insecticidal proteins were fractionated and enriched as described. For identification candidate protein bands were excised, digested with trypsin and analyzed by nano-liquid chromatography/electrospray tandem mass spectrometry (nano-LC/ESI-MS/MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific) interfaced with an Eksigent® NanoLC-1D™ Plus nanoLC™ system (AB Sciex). Ten product ion spectra were collected in an information dependent acquisition mode after a MS1 survey scan.

Protein identification was done by database searches using Mascot (Matrix Science). The searches were done against the in-house database Bacteria-Plus, which combines all bacterial protein sequences and keratin sequences derived from the NCBI non-redundant database (nr) as well as in-house protein sequences.

US11780891B2. Insecticidal proteins and methods for their use (2023-10-10). PIONEER HI-BRED INTERNATIONAL, INC. [US]. Inventors: Steven D Gruver [US], Heather Kozy [US], Jessica O'Rear [US], Barbara Rosen [US], Ute Schellenberger [US], Jun-Zhi Wei [US], Weiping Xie [US], Xiaohong Zhong [US], Genhai Zhu [US].
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2

Protein Identification by Tandem Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

All insecticidal proteins were fractionated and enriched as described. For identification candidate protein bands were excised, digested with trypsin and analyzed by nano-liquid chromatography/electrospray tandem mass spectrometry (nano-LC/ESI-MS/MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific) interfaced with an Eksigent® NanoLC-1D™ Plus nanoLC™ system (AB Sciex). Ten product ion spectra were collected in an information dependent acquisition mode after a MS1 survey scan.

Protein identification was done by database searches using Mascot (Matrix Science). The searches were done against the in-house database Bacteria-Plus, which combines all bacterial protein sequences and keratin sequences derived from the NCBI non-redundant database (nr) as well as in-house protein sequences.

US10820596B2. Insecticidal proteins and methods for their use (2020-11-03). PIONEER HI-BRED INTERNATIONAL, INC. [US]. Inventors: Steven D Gruver [US], Heather Kozy [US], Jessica O'Rear [US], Barbara Rosen [US], Ute Schellenberger [US], Jun-Zhi Wei [US], Weiping Xie [US], Xiaohong Zhong [US], Genhai Zhu [US].
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3

Protein Identification by Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

All insecticidal proteins were fractionated and enriched as described. For identification candidate protein bands were excised, digested with trypsin and analyzed by nano-liquid chromatography/electrospray tandem mass spectrometry (nano-LC/ESI-MS/MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific) interfaced with an Eksigent® NanoLC-1D™ Plus nanoLC™ system (AB Sciex). Ten product ion spectra were collected in an information dependent acquisition mode after a MS1 survey scan.

Protein identification was done by database searches using Mascot (Matrix Science). The searches were done against the in-house database Bacteria-Plus, which combines all bacterial protein sequences and keratin sequences derived from the NCBI non-redundant database (nr) as well as in-house protein sequences.

US11203619B2. Insecticidal proteins and methods for their use (2021-12-21). PIONEER HI-BRED INTERNATIONAL, INC. [US]. Inventors: Steven D Gruver [US], Heather Kozy [US], Jessica O'Rear [US], Barbara Rosen [US], Ute Schellenberger [US], Jun-Zhi Wei [US], Weiping Xie [US], Xiaohong Zhong [US], Genhai Zhu [US].
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4

Protein Identification via Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

All insecticidal proteins were fractionated and enriched as described. For identification candidate protein bands were excised, digested with trypsin and analyzed by nano-liquid chromatography/electrospray tandem mass spectrometry (nano-LC/ESI-MS/MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific) interfaced with an Eksigent® NanoLC-1D™ Plus nanoLC™ system (AB Sciex). Ten product ion spectra were collected in an information dependent acquisition mode after a MS1 survey scan.

Protein identification was done by database searches using Mascot (Matrix Science). The searches were done against the in-house database Bacteria-Plus, which combines all bacterial protein sequences and keratin sequences derived from the NCBI non-redundant database (nr) as well as in-house protein sequences.

US10448648B2. Insecticidal proteins and methods for their use (2019-10-22). PIONEER HI-BRED INTERNATIONAL, INC. [US]. Inventors: Steven Gruver [US], Heather Kozy [US], Jessica O'Rear [US], Barbara Rosen [US], Ute Schellenberger [US], Jun-Zhi Wei [US], Weiping Xie [US], Xiaohong Zhong [US], Genhai Zhu [US].
+ Open protocol
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5

Protein Identification by Tandem Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

All insecticidal proteins were fractionated and enriched as described. For identification candidate protein bands were excised, digested with trypsin and analyzed by nano-liquid chromatography/electrospray tandem mass spectrometry (nano-LC/ESI-MS/MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific) interfaced with an Eksigent® NanoLC-1D™ Plus nanoLC™ system (AB Sciex). Ten product ion spectra were collected in an information dependent acquisition mode after a MS1 survey scan.

Protein identification was done by database searches using Mascot (Matrix Science). The searches were done against the in-house database Bacteria-Plus, which combines all bacterial protein sequences and keratin sequences derived from the NCBI non-redundant database (nr) as well as in-house protein sequences.

US10231460B2. Insecticidal proteins and methods for their use (2019-03-19). PIONEER HI-BRED INTERNATIONAL, INC. [US]. Inventors: Steven Gruver [US], Heather Kozy [US], Jessica O'Rear [US], Barbara Rosen [US], Ute Schellenberger [US], Jun-Zhi Wei [US], Weiping Xie [US], Xiaohong Zhong [US], Genhai Zhu [US].
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