488 rabbit

Immunohistochemical analyses were performed as previously described (Colombo et al., 2006 (link)). The primary antibodies utilized were the following: anti-CTIP2 (Abcam ab18465), anti-CUX1 (santa Cruz Biotechnology sc-13024), anti-SATB2 (Abcam, ab51502), anti-PAX6 (Covance #PRB-278P), anti GFAP (chicken, 1:1,000, Abcam, ab4674), anti-DCX (rabbit, 1:1,000, Abcam, ab18723), antiKI67 (rabbit, 1:500, immunological sciences, mab-90948), anti-NEUN (rabbit, 1:500, Abcam, ab104225).
Secondary antibodies: 488-mouse (donkey, 1:2,000, Molecular Probes, A21202), 488-rabbit (donkey, 1:2,000, Molecular Probes, A21026), 594-mouse (donkey, 1:2,000, Molecular Probes, A10036), 594-rabbit (donkey, 1:2,000, Molecular Probes, A21207). DAPI (4′,6′-diamidino-2-phenylindole) was used as nuclear counterstaining.

Immunocytochemicalstaining was performed as previously described (Sessa et al., 2019 (link)).
The primary antibodies utilized were the following: anti-KI67 (Immunological Science, MAB90948), anti-GFAP (Merck, AB5804), anti-TUJ1 (Covance, MRB-435P), anti-phospho histone 3 (PH3) (rabbit, 1:400, Sigma-Aldrich, H0412), anti-S100b (Dako, GA504). Anti-O4 primary antibody was produced from a hybridoma clone, using the culture media of hybridoma cells directly on living cells to perform the staining for O4 epitope. Secondary antibody used: 488-mouse (donkey, 1:2,000, Molecular Probes, A21202), 488-rabbit (donkey, 1:2,000, Molecular Probes, A21026), 594-mouse (donkey, 1:2,000, Molecular Probes, A10036), 594-rabbit (donkey, 1:2,000, Molecular Probes, A21207). DAPI (4′,6′-diamidino-2-phenylindole) was used as nuclear counterstaining.
The quantification of Radial Glia cells in the hippocampus has been performed using GFAP antibody, counting only the GFAP⁺ cells present in the subgranular zone of the dentate gyrus.