Analytical grade reagents were required in the procedures. n-Hexane (96%) and methanol (MeOH, ≥99%) for HPLC gradient quality were supplied by Scharlab (Barcelona, Spain). Sodium hydroxide pellets (NaOH) (98%), sulphuric acid (H2SO4, 98%), boric acid (≥99%) and methylene blue (82%) were obtained from Panreac (Barcelona, Spain). Dimethyl sulfoxide (DMSO, ≥99.9%) and 2,2-diphenyl-1-picrylhydrazyl (DPPH, ≥99.9%) were provided by Sigma-Aldrich (St. Louis, MO, USA). Methyl red, Kjeldahl catalyst tables and distilled water system were purchased from Merck (Madrid, Spain). Fatty acid standards FAME 37 component SUPELCO Ref CRM47885, PUFA No. 3 Menhaden oil Ref 47085-U and tridecanoic acid (C13:0, ≥ 99%) were purchased from Sigma-Aldrich (Barcelona, Spain). The standard Trolox was provided by Sigma-Aldrich (Burghasen, Germany).
Sodium hydroxide pellets
Manufactured by ITW Reagents
Sourced in Spain
Sodium hydroxide pellets are a chemical compound with the chemical formula NaOH. They are a white, solid, and crystalline material that is highly soluble in water. Sodium hydroxide pellets are commonly used as a pH adjusting agent in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Sulphuric acid, by ITW Reagents (2 mentions)
Trolox, by Merck Group (1 mentions)
Methyl red, by Merck Group (1 mentions)
Tridecanoic acid, by Merck Group (1 mentions)
Methanol, by Scharlab (1 mentions)
Methylene blue, by ITW Reagents (1 mentions)
Boric acid, by ITW Reagents (1 mentions)
N hexane, by Scharlab (1 mentions)
Sodium carbonate anhydrous, by ITW Reagents (1 mentions)
Milli q system, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Rutin trihydrate, by Merck Group (1 mentions)
Myricetin, by Merck Group (1 mentions)
Gallic acid monohydrate, by Merck Group (1 mentions)
7 protocols using sodium hydroxide pellets
1
Analytical Reagents for Comprehensive Analysis
2
Quantification of Phenolic Compounds
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Analytical-grade reagents and purified water from a Milli-Q system (Merck, Madrid, Spain) were employed in all procedures. Sodium carbonate anhydrous, aluminum chloride 6-hydrate, sodium hydroxide pellets, and sodium nitrite were supplied by Panreac (Barcelona, Spain). Dimethyl sulfoxide (DMSO, ≥99.9%), 2 N Folin-Ciocalteu reagent, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ethanol (EtOH), acetonitrile (ACN), methanol (MeOH), and formic acid (FA) of MS quality were provided by Scharlab (Barcelona, Spain). The phenolic standards, dihydroxy-benzoic acid (≥97.0%), caffeic acid (≥98.0%), p-coumaric acid (≥98.0%), trans-ferulic acid (98%), gallic acid monohydrate (≥98.0%), kaempferol (≥97.0%), myricetin (≥96.0%), quercetin (≥95.0%), rutin trihydrate (≥95.0%), and resveratrol (≥99.0%) were supplied by Sigma-Aldrich (Madrid, Spain). The analytical standard, hesperidin (≥98.0%) was provided by European Pharmacopoeia. Phenolic stock solutions (200 mg·L−1) were prepared in MeOH, 80:20 (v/v) ethanol-water mixture (for quercetin), or 5% (v/v) DMSO aqueous solution (for hesperidin). These solutions were stored in the dark at 4 °C or −80 °C for up to two months (of myricetin, hesperidin, trans-ferulic, and caffeic acid). Fresh working standard solutions were prepared daily by diluting the stock solutions as required.
3
Hydrogel Scaffold Fabrication
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Sodium alginate from brown algae (SA, low viscosity); propargylamine (PA, 98%); N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, ≥98%); N-hydroxysuccinimide (NHS); 1,4-dithiothreitol (DTT); poly(ethylene glycol) dithiol (PEG-SH, average Mn 1000); lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Z95%); and hydrochloric acid solution (37%) were all purchased from Sigma-Aldrich and used as received without further purification. Sodium hydroxide pellets were purchased from Panreac, and pre-wetted dialysis membranes (MWCO 3500 Da Spectra/Por6) from Spectrum Laboratories.
4
Quantification of Carbohydrate Derivatives
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HPLC-grade d (+)-glucose, d (+)-galactose, d (+)-xylose, l (+)-arabinose, d (+)-mannose, formic acid, acetic acid and furfural were from Panreac (Barcelona, Spain). Levulinic acid was from Fluka Analytical-Sigma Aldrich (Steinheim, Germany). 5-Hydroxymethyl-2-furaldehyde (HMF) and l (−)-fucose were from Sigma Aldrich (Steinheim, Germany). Sodium hydroxide pellets and sulphuric acid were from Panreac (Barcelona, Spain).
5
Cellulose-Chitosan Nanocomposite Synthesis
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Commercial cotton linters supplied by Sigma Aldrich were utilized as a cellulose source to produce CCNC. The chemicals applied in the different steps of the synthesis and characterization processes were sodium (meta)periodate, Girard’s Reagent-T ((2-hydrazinyl-2-oxoethyl)-trimethylazanium chloride, GT), ethylene glycol, hydroxylamine hydrochloride, silver nitrate, potassium peroxodisulfate, sodium chloride and hydrochloric acid (37% v/v) which were supplied by Sigma Aldrich (St. Louis, MO, USA) and sodium hydroxide pellets supplied by Panreac (Barcelona, Spain). All the purchased chemicals were of analytical grade.
Standard solutions of hexavalent chromium (50 mg·L−1) were used as calibration standards for the spectrophotometric analysis supplied by Hach. Hexavalent chromium in wastewater samples was analyzed following Standard Method 3500-Cr-B [24 ] through the analytical reagent kits supplied by Macherey Nagel (Düren, Germany). Poly(diallyldimethylammonium chloride) (PDADMAC) and polyethylenesulfonate (PesNA) standard solutions with 2.5·10−4 eq·L−1 of concentration were applied as titration reagents to determine cationic and anionic demand.
Standard solutions of hexavalent chromium (50 mg·L−1) were used as calibration standards for the spectrophotometric analysis supplied by Hach. Hexavalent chromium in wastewater samples was analyzed following Standard Method 3500-Cr-B [24 ] through the analytical reagent kits supplied by Macherey Nagel (Düren, Germany). Poly(diallyldimethylammonium chloride) (PDADMAC) and polyethylenesulfonate (PesNA) standard solutions with 2.5·10−4 eq·L−1 of concentration were applied as titration reagents to determine cationic and anionic demand.
6
Quantitative Analysis of Olive Oil Compounds
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Cyclohexane and 0.1 N sodium thiosulfate (Na2S2O3) was purchased from Carlo Erba Reagents (Val-de-Reuil, France); acetic acid, chloroform, methanol (MeOH), and acetonitrile (ACN) from Sigma-Aldrich (Madrid, Spain); potassium iodide (KI) from Honeywell Fluka (Buchs, Switzerland); hexane, sodium hydroxide pellets (NaOH), starch 1% and phenolphthalein from Panreac (Castellar del Vallès, Spain), and ethanol 96% from VWR Chemicals (Fontenay-sous-Bois, France). Ultrapure water was obtained using a Milli-Q purification system (Millipore, Bedford, MA, USA).
Regarding the standards, oleocanthal (≥95% purity) was purchased from Merck (Darmstadt, Germany); oleacein and oleuropein aglycone (≥90% and 95% purity, respectively) from Toronto Research Chemical Inc. (ON, Canada). Luteolin (≥96% purity), oleuropein (98% purity), pinoresinol (≥95% purity), squalene, and (±)-α-tocopherol (≥96% purity) were acquired from Sigma-Aldrich. Apigenin and p-coumaric (>98% purity) were from Fluka, and hydroxytyrosol was from Extrasynthese (Genay, France).
Regarding the standards, oleocanthal (≥95% purity) was purchased from Merck (Darmstadt, Germany); oleacein and oleuropein aglycone (≥90% and 95% purity, respectively) from Toronto Research Chemical Inc. (ON, Canada). Luteolin (≥96% purity), oleuropein (98% purity), pinoresinol (≥95% purity), squalene, and (±)-α-tocopherol (≥96% purity) were acquired from Sigma-Aldrich. Apigenin and p-coumaric (>98% purity) were from Fluka, and hydroxytyrosol was from Extrasynthese (Genay, France).
7
Biodegradable Polymer Composite Synthesis
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High-amylose corn starch (amylose content ≥ 80%) was kindly supplied by Roquette (Roquette Laisa España, Benifaio, Spain) and glycerol was purchased from Panreac Quimica, S.A. Castellar Del Vallés, Barcelona, Spain. Poly(3-hydroxybutyrate) (PHB) was supplied by Biomer (Krailling, Germany). 2,2,2-Trifuoroethanol (TFE) with 99 % purity was purchased from Sigma-Aldrich (Spain). All products were used as received without further purification. Sulphuric acid 96 wt% and sodium hydroxide pellets were purchased from Panreac (Barcelona, Spain). The bacteria strain G. xylinus was obtained from the Spanish type culture collection (CECT).
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