Chlorin e6 was purchased from J&K Scientific Ltd. (Beijing, China). Diclofenac, morpholin-4-yl-acetic acid hydrochloride, 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride (EDC), 4-dimethylaminopyridine (DMAP) and trimethylamine (TEA) were purchased from Aladdin (Shanghai, China). Bilirubin and N,N′-diisopropylcarbodiimide (DIC) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). mPEG2k-NH2 and Fmoc-NH-PEG2k-NH2 were purchased from Ponsure Biotechnology (Shanghai, China). Cou-6 was obtained from Sigma–Aldrich (USA). DiD and DCFH-DA were obtained from Meilunbio (Dalian, China). The annexin V-fluoresceine isothiocyanate (FITC) apoptosis detection kit and calcium-AM were obtained from Yeason (Shanghai, China). Lactate detection kit and lactate dehydrogenase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Naijing, China). Anti-HIF-1α antibody, Cy3 goat anti-rabbit IgG (H + L) and Alexa fluor 647 goat anti-rabbit IgG (H + L) secondary antibody were purchased from Abcam (Hongkong, China). Anti-LDHA and anti-CD31 antibody were purchased from Servicebio Company (Wuhan, China). Anti-LDHB and anti-c-MYC antibody were purchased from Proteintech (Wuhan, China). Anti-VEGFα and anti-angiopoietin-2 antibody were purchased from Abclonal Company (Wuhan, China).
Anti ldha
Manufactured by Wuhan Servicebio Technology
Sourced in China
Anti-LDHA is a laboratory reagent used for the detection and quantification of the LDHA (Lactate Dehydrogenase A) protein. LDHA is an enzyme involved in the conversion of pyruvate to lactate during anaerobic glycolysis. The Anti-LDHA reagent can be used in various research applications to study LDHA expression and activity.
Automatically generated - may contain errors
Lab products found in correlation
Lactate dehydrogenase assay kit, by Nanjing Jiancheng (1 mentions)
Bilirubin, by Tokyo Chemical Industry (1 mentions)
Anti cd31 antibody, by Wuhan Servicebio Technology (1 mentions)
Anti hif 1α antibody, by Abcam (1 mentions)
Dcfh da, by Meilun (1 mentions)
N n diisopropylcarbodiimide, by Tokyo Chemical Industry (1 mentions)
Anti ldhb, by Proteintech (1 mentions)
Chlorin e6, by J&K Scientific (1 mentions)
Anti c myc antibody, by Proteintech (1 mentions)
Cou 6, by Merck Group (1 mentions)
Lactate detection kit, by Nanjing Jiancheng (1 mentions)
Ecl reagent, by Thermo Fisher Scientific (1 mentions)
Bca protein assay reagent, by Beyotime (1 mentions)
Anti rabbit secondary antibody, by Beyotime (1 mentions)
Membrane and cytosol protein extraction kit, by Beyotime (1 mentions)
Anti ido1, by Proteintech (1 mentions)
Anti β actin, by Abways (1 mentions)
Anti mouse secondary antibody, by Beyotime (1 mentions)
Anti bax, by Wuhan Servicebio Technology (1 mentions)
Anti bcl 2, by ABclonal (1 mentions)
3 protocols using anti ldha
1
Chlorin e6-Mediated Photodynamic Therapy
2
Immunohistochemical Analysis of LDHA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin‐embedded tissue sections underwent dewaxing, rehydration, and antigen retrieval using citrate buffer. We then quenched endogenous peroxidase activity and blocked nonspecific binding with hydrogen peroxide and serum. The slides were incubated with anti‐LDHA (1:200; Servicebio; Cat number: GB11342) antibody overnight at 4°C. This was followed by the application of a secondary antibody from goat anti‐rabbit (Beyotime), visualization with DAB, and hematoxylin counterstaining. The H score was calculated as previously described.
58 (link)
58 (link)
3
Western Blot Analysis of Cellular Fractions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC cells were lysed using the RIPA lysis buffer (Beyotime, China; Cat number: P0013B), and supernatants were collected as total protein. The PM fraction of cells was isolated following the standard procedure of membrane and cytosol protein extraction kit (Beyotime; Cat number: P0033). The protein concentration was assessed using the BCA Protein Assay Reagent (Beyotime; Cat number: P0012S). Lysates were subjected to 10% SDS‐PAGE and transferred onto PVDF membranes. Following blocking with PBS containing 5% nonfat milk, membranes were incubated with the respective primary antibodies: anti‐IDO1 (1:5000; Proteintech, USA; Cat number: 66528‐1‐Ig), anti‐HK2 (1:1000; Servicebio, China; Cat number: GB111063), anti‐LDHA (1:2000; Servicebio; Cat number: GB11342), anti‐GLUT1 (1:1000; Servicebio; Cat number: GB113495), anti‐Na+/K+ ATPase (1:1000; Beyotime; Cat number: AF1864), anti‐Bax (1:800; Servicebio; Cat number: GB114122), anti‐Bcl‐2 (1:1000; ABclonal, China; Cat number: A19693), or anti‐β‐actin (1:5000; Abways Technology, China; Cat number: AB2001) antibodies. After incubation with an HRP‐conjugated anti‐mouse secondary antibody (Beyotime) or anti‐rabbit secondary antibody (Beyotime), the proteins were detected using ECL reagents (Thermo Fisher Scientific, USA) and immunoreactive signals were quantitatively assessed using densitometry.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!