Anti β actin and anti lamin b1 antibodies

Efonidipine was purchased from Selleckchem (Houston, TX, USA). SP600125 was purchased from Calbiochem (Darmstadt, Germany). LPS (Escherichia coli, serotype O111:B4), dimethyl sulfoxide (DMSO), and anti-β-actin and anti-lamin B1 antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) and Alexa Fluor 488-conjugated anti-mouse immunoglobulin G were purchased from Thermo Fisher Scientific (Rockford, IL, USA). Fluorescent mounting medium was obtained from Dako (Carpinteria, CA, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and antibiotic-antimycotic reagent were obtained from Corning Life Sciences (Corning, NY, USA). The anti-phospho-inhibitory kappa Bα (IκBα) antibody was obtained from Abcam (Cambridge, MA, USA). Primary antibodies specifically recognizing phospho-JNK, JNK3, phospho-c-Jun, c-Jun, inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB) p65, and IκBα, and horseradish peroxidase (HRP)-conjugated secondary anti-rabbit and anti-mouse antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).