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Goat anti mouse igg hrp sc 2302

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Goat anti-mouse IgG-HRP (sc-2302) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) for use in various immunoassay and immunoblotting techniques.

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2 protocols using goat anti mouse igg hrp sc 2302

1

NF-κB Pathway Protein Detection

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RelB (BB-AB0076C), p50 (BB-AB0080C), p52 (AB0085C), H3 (BB-AB0055C), tubulin (BB-AB0118C), GAPDH (BB-AB0060) antibodies and Protein A PLUS agarose bead (BB-PA001PE) were purchased from BioBharati LifeScience (BBL), Kolkata, India. NF-κB p65/RelA (D14E12) XP antibody and HA (3,724) antibody were purchased from Cell Signaling Technology. Bcl3 (23959-1-AP-100UL) antibody was purchased from Proteintech. Flag M2 (F3165) antibody was purchased from Sigma-Aldrich. Goat anti-mouse IgG-HRP (sc-2302) and goat anti-rabbit IgG-HRP (sc-2030) were purchased from Santa Cruz. The dual-luciferase reporter assay system (E1910) was purchased from Promega. The SuperSignal West Pico Chemiluminescent Substrate kit (34,580) was purchased from ThermoFisher.
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2

Antibody Characterization for EMT and Autophagy

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Primary antibodies directed against AEG-1/lyric/metadherin (CST-9596), MAP-LC3 (CST-12741), TGF-β1 (CST-3711), LAMP-1 (CST-9091) and BECN1 (CST-4122) are purchased from Cell Signaling Technology, Inc. (Beverly, MA). Primary antibodies directed against E-cadherin (BS-1097), N-cadherin (BS-2224), vimentin (BS-1855), mTOR (BS-3611) and p-mTOR (BS-4706) are purchased from Bioworld Technology Co., Ltd. Primary antibody directed against β-actin (BM0627) is purchased from Boster Biological Technology, Ltd. Primary antibodies directed against AEG-1/MTDH (13860-1-AP) Snail (26183-1-AP), Twist1 (25465-1-AP), Slug (12129-1-AP) are purchased from Proteintech Technology, Inc. (Wuhan, China). Primary antibodies against Smad2/3 (sc-6202), p-Smad2/3 (Ser 423/425) (sc-11769) and Smad2/3 siRNA (sc-37238) are purchased from Santa Cruz Technology, Ltd. (Santa Cruz, CA). The secondary antibodies used are as follows: (1) anti-mouse IgG: IRDye 800-conjugated anti-mouse IgG (Rockland, Inc., Philadelphia, PA); (2) anti-rabbit IgG: Alexa Fluor 680 goat anti-rabbit IgG (Invitrogen, Carlsbad, CA); (3) anti-goat IgG: Alexa Fluor 680 rabbit anti-goat IgG (Invitrogen, Carlsbad, CA); (4) goat anti-mouse IgG HRP (SC-2302, Santa Cruz Biotechnology, Inc., Texas, USA); (5) goat anti-rabbit IgG HRP (SC-2030, Santa Cruz Biotechnology, Inc., Texas, USA).
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