100 µL of suspended cells was transferred to black flat-bottomed 96-well microtiter plates (Greiner Bio-One GmbH, Frickenhausen, Deutschland) and supplemented with 10 µM fluorogen of the green dye TFLime (ex. 480/em. 541) or the red dye TFCoral (ex. 516/em. 600) (The Twinkle Factory, France, Paris). Excitation and emission wavelengths both correspond to the respective maximum of the fluorogen. Fluorescence intensities of the whole population were determined using the SYNERGY H1 microplate reader (BioTek, Bad Friedrichshall, Germany) located in an anaerobic chamber and finally normalized to the OD600 of PBS-washed cells.
Tfcoral
Manufactured by Greiner
TFCoral is a laboratory equipment product designed for use in scientific research and analysis. It functions as a tool for the manipulation and processing of samples in a controlled environment. The core purpose of TFCoral is to facilitate the examination and study of various materials and substances.
Automatically generated - may contain errors
2 protocols using tfcoral
1
Live-cell Fluorescence Quantification
2
Quantifying Fluorescence of FAST-tagged Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescence of FAST and FAST-tagged fusion protein-producing recombinant E. limosum strains, cultivated anaerobically until the stationary growth phase, were determined using a microplate reader, uorescence microscopy, or ow cytometry. Therefore, 2 mL culture broth was taken anaerobically during growth and harvested by centrifugation at 7,711 x g for 15 minutes at 4°C. The supernatant was discarded and harvested cells were washed with anaerobic PBS buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 )
followed by centrifugation at 7,711 x g for 15 minutes at 4°C. Cell pellets were suspended in anaerobic PBS buffer (end OD 600 1).
Microplate reader 100 µL of suspended cells were transferred to black at bottomed 96-well microtiter plates (Greiner Bio-One GmbH, Frickenhausen, Deutschland) and supplemented with 10 µM uorogen of the green dye TF Lime (ex. 480/em. 541) or the red dye TF Coral (ex. 516/em. 600) (The Twinkle Factory, France, Paris). Excitation and emission wavelengths both correspond to the respective maximum of the uorogen. Fluorescence intensities of the whole population were determined using the SYNERGY H1 microplate reader (BioTek, Bad Friedrichshall, Germany) located in an anaerobic chamber and nally normalized to the OD 600 of PBS-washed cells.
followed by centrifugation at 7,711 x g for 15 minutes at 4°C. Cell pellets were suspended in anaerobic PBS buffer (end OD 600 1).
Microplate reader 100 µL of suspended cells were transferred to black at bottomed 96-well microtiter plates (Greiner Bio-One GmbH, Frickenhausen, Deutschland) and supplemented with 10 µM uorogen of the green dye TF Lime (ex. 480/em. 541) or the red dye TF Coral (ex. 516/em. 600) (The Twinkle Factory, France, Paris). Excitation and emission wavelengths both correspond to the respective maximum of the uorogen. Fluorescence intensities of the whole population were determined using the SYNERGY H1 microplate reader (BioTek, Bad Friedrichshall, Germany) located in an anaerobic chamber and nally normalized to the OD 600 of PBS-washed cells.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!