A Superose 6 (10/300) column was equilibrated with buffer consisting of 25 mM Tris-HCl pH 7.9, 5 mM MgCl2, 150 mM KCl, 5% Glycerol, 1 mM DTT and 1× PIC (Roche). Five hundred μL of whole-cell extracts containing ∼5 mg of protein were injected in an ÄKTA avant chromatography system (Cytiva) and run at 0.4 mL/min. Protein detection was performed by absorbance at 280 nm and 260 nm. Five hundred μL fractions were collected and analysed by western blot and IP-MS.
Kta avant chromatography system
Manufactured by Cytiva
The ÄKTA avant chromatography system is a versatile and high-performance liquid chromatography instrument designed for a wide range of applications. It is capable of performing various separation techniques, including ion exchange, size exclusion, and affinity chromatography. The system is equipped with advanced software and hardware features that enable precise control and monitoring of the chromatographic process. The ÄKTA avant is suitable for use in research and development laboratories, as well as in biopharmaceutical manufacturing environments.
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Lab products found in correlation
2 protocols using kta avant chromatography system
1
Purification of Protein Complexes
2
Protein Purification and Identification
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A Superose 6 (10/300) column was equilibrated with buffer consisting of 25mM Tris HCl pH 7.9, 5 mM MgCl2, 150 mM KCl, 5% Glycerol, 1 mM DTT and 1x PIC (Roche). Five hundred μL of whole cell extracts containing ∼5 mg of protein were injected in an ÄKTA avant chromatography system (Cytiva) and run at 0.4 mL/min. Protein detection was performed by absorbance at 280 nm and 260 nm. Five hundred μL fractions were collected and analysed by Western blot and IP-MS.
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