Ct26 wt atcc crl 2638

CT26.WT (ATCC^® CRL-2638™) was obtained from American Type Culture Collection (ATCC). This is an N-nitroso-N-methylurethane-(NNMU) induced murine colorectal cell line syngeneic with Balb/c mice. This cell line shares molecular features with sporadic, aggressive, undifferentiated (stage IV), therapy-refractory human colorectal carcinoma cells, it is easily implanted and metastasizes readily (Castle et al., 2014 (link)). The cells were propagated, screened for mycoplasma contamination (MycoAlert™ PLUS mycoplasma detection kit, Lonza Walkersville, Walkersville, MD) and subcultured in accordance to the distributor’s protocol. Cells were grown in RPMI-1640 medium with 10% FBS, penicillin (100 U/ml) and streptomycin (100 μg/ml) and maintained at 37°C with 5% CO₂ in a humidified atmosphere. After harvesting and preparation of cells, their total number and viability were determined by counting in a Neubauer chamber using Trypan Blue Dye, and was always found to be at least 95% viable.

CT26.WT (ATCC^® CRL-2638™) was obtained from American Type Culture Collection (ATCC). This is an N-nitroso-N-methylurethane-(NNMU) induced murine colorectal cell line syngeneic with Balb/c mice. This cell line shares molecular features with sporadic, aggressive, undifferentiated (stage IV), therapy-refractory human colorectal carcinoma cells, it is easily implanted and metastasizes readily [98 (link)]. The cells were propagated and subcultured in accordance to the distributor’s protocol, i.e., they were grown in RPMI-1640 medium with 10% FBS, penicillin (100 U/mL) and streptomycin (100 μg/mL) and maintained at 37 °C with 5% CO₂ in a humidified atmosphere. After harvesting and preparation of cells, their total number and viability were determined by counting in a Neubauer chamber using Trypan Blue Dye, and was always found to be at least 95%.