Plasma was collected by rapid retro-orbital blood collection with hematocrit tubes. Plasma aldosterone levels were determined using an aldosterone I125 radioimmunoassay (Tecan, Morrisville, NC, MG13051). Plasma renin activity was determined on EDTA-collected plasma using a competitive radioimmunoassay for Angiotensin I following the manufacturer’s instructions (Tecan, Morrisville, NC, 30120823). To rule out the influence of limiting amounts of angiotensinogen substrate in the plasma, an excess (about 180 ng) of angiotensinogen 1–14 renin substrate porcine (Sigma, SCP0021) was added to every sample, calibrator and control right before quantification. Gamma counts were measured for 1 min.
Scp0021
Manufactured by Merck Group
The SCP0021 is a laboratory instrument designed for performing spectrophotometric analysis. It is capable of measuring the absorbance of light by a sample across a range of wavelengths. The core function of this product is to provide accurate and reliable data on the optical properties of various substances, which can be used for a variety of scientific and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using scp0021
1
Plasma Aldosterone and Renin Assay
2
Plasma Renin Activity Measurement in Olfr558 Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma renin activity was measured in 3-month-old Olfr558 WT and KO mice with a modified angiotensin I measurement kit (S-1188, Peninsula Laboratories). Plasma was collected from male and female Olfr558 WT and KO mice treated with 0.49% NaCl diet (catalog no. TD.96208 modified with orange color, Envigo). From males, we also collected plasma from mice given Teklad diet (Teklad 2018SX, 18% protein). Plasma was diluted 15-fold and then incubated with excess porcine angiotensinogen (SCP0021, Sigma-Aldrich) for 20 min at 37°C in a buffer containing 50 mM sodium acetate (pH 6.5), 10 mM 4-(2-aminoethyl) benzenesulfony fluoride hydrochloride (Sigma-Aldrich), 10 mM EDTA (pH 8.0), 1 μM porcine angiotensinogen, and 10 mM 8-hydroxyquinoline (Sigma-Aldrich). After incubation, the sample was analyzed according to the provided protocol. Plasma renin activity was assayed by competitive binding of angiotensin I antibody.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!