Nta resins

Manufactured by Qiagen

Sourced in Germany

NTA (Nitrilotriacetic acid) resins are solid-phase affinity media used for the purification of recombinant proteins or other biomolecules. They bind to histidine-tagged proteins, allowing for selective capture and subsequent elution of the target protein. These resins provide a convenient and efficient way to isolate proteins of interest from complex mixtures.

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Lab products found in correlation

Protease inhibitor cocktail, by Merck Group (1 mentions)

Close spelling variants of this product

Ni-NTA fast flow resin Ni-NTA Superflow affinity resin NTA)-resin chromatography Nickel-nitrilotriacetic acid (Ni-NTA) resin Ni–NTA chromatography resin Ni-NTA agarose superflow resin NTA-resin affinity chromatography Ni2+-nitrilotriacetic acid (NTA) resin Ni-NTA) affinity resin column Ni2+-charged NTA resin

2 protocols using nta resins

Affinity Purification of GST-tagged Proteins

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Glutathione S-transferase (GST), GST-dArab11, GST-dNrab11, GST-rab1-His, GST-rab5-His, or GST-rab11-His proteins were affinity-purified from bacteria with appropriate resins according to the manufacturer’s instructions. Proteins tagged with both GST and 6×His were sequentially purified first with Ni–nitrilotriacetic acid (NTA) resins (Qiagen) and then with glutathione resins. All purified proteins were dialyzed and frozen in aliquots at −80°C until use.

Ke Y., Weng M., Chhetri G., Usman M., Li Y., Yu Q., Ding Y., Wang Z., Wang X., Sultana P., DiFiglia M, & Li X. (2020). Trappc9 deficiency in mice impairs learning and memory by causing imbalance of dopamine D1 and D2 neurons. Science Advances, 6(47), eabb7781.

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Protein Extraction and Immunoprecipitation

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Cells were lysed in 50 mM Tris-HCl (pH 8.0) containing 150 mM NaCl, 0.5% NP-40, 1 mM PMSF, tyrosine protein phosphatase inhibitor (Sigma St. Louis, Missouri, USA), and 1x protease inhibitor cocktail (Sigma). Cell lysates were incubated with indicated antibodies for 2 hours at 4°C and then with 30 μl of 50% slurry of protein A-conjugated Sepharose for the next 1 hour. For pull-down analysis, cell lysates were prepared as above and treated with NTA resins (Qiagen Hilden, Germany). Cell lysates were incubated with indicated antibodies for 2 hours at 4°C and then with 50 μl of 50% slurry of protein A/G-conjugated dynabeads for the next 10 minutes.

Park J.M., Yang S.W., Zhuang W., Bera A.K., Liu Y., Gurbani D., von Hoyningen-Huene S.J., Sakurada S.M., Gan H., Pruett-Miller S.M., Westover K.D, & Potts M.B. (2021). The nonreceptor tyrosine kinase SRMS inhibits autophagy and promotes tumor growth by phosphorylating the scaffolding protein FKBP51. PLoS Biology, 19(6), e3001281.

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