Ft nmr avance 3 hd 600 mhz spectrometer

FTIR spectra were determined on lyophilized tissues according to the method reported in the paragraph 2.1.1. Nuclear magnetic resonance (NMR) spectra were recorded with a Bruker FT-NMR AVANCE III HD 600 MHz spectrometer at 298 K; the Larmor frequencies of proton and carbon were 600.13 MHz and 150.90 MHz for ¹H and ¹³C, respectively. Each sample was acquired in both solid state and in semi-solid state, using respectively a 2.5 mm double resonance H-X CP-MAS probe and 4 mm ¹H/¹³C/³¹P HR MAS probe equipped with ²H lock and Z gradients.

One-dimensional ¹H NMR spectra of juice mixtures were acquired with a Bruker FT-NMR Avance III HD 600 MHz spectrometer (Ettlingen, Germany), and all the NMR experiments were performed at 300 °K. After 10 min of thermal equilibration inside the probe, the solvent (D₂O) was locked to assure the maximum sensitivity, the probe was manually tuned and matched, and the 90° pulse was calibrated; subsequently, the power level for pre-saturation was calculated, and finally the receiver gain was automatically set. 1D NOESY experiments were acquired by using the Bruker sequence “noesygppr1d” in order to suppress the water signal. The pre-saturation frequency was adjusted for each sample before acquisition. The acquisition parameters were as follows: time domain (number of data points), 64 K; dummy scans, 0; number of scans, 32; acquisition time, 3.90 s; delay time, 8 s; spectral width, 14 ppm (8403.4 Hz), fid resolution, 0.2564 Hz; digitization mode, baseopt, total acquisition time, 6 min 22 s.