Poly(ε-caprolactone) (PCL, [η] = 1.8 ± 0.2 dL/g) was purchased from Polymtek (Shenzhen, China). Pluronic® F127 (F127, molecular weight) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The ropivacaine base (Rop), clonidine hydrochloride (Clo), and lipase were purchased from Macklin (Shanghai, China). Ropivacaine hydrochloride solution (Rop-HCl) was obtained from AstraZeneca AB (SE-151 85 Sodertalje, Sweden). Fluorescein isothiocyanate (FITC), Rhodamine B (RhB), dichloromethane (DCM), methanol, and acetone were purchased from Aladdin (Shanghai, China). Toluidine blue O (TBO) dye, TUNEL apoptosis assay kit, diaminobenzidine (DAB), TNF-α, and IL-6 rabbit pAb were purchased from Servicebio (Wuhan, China).
Tunel apoptosis assay kit
Manufactured by Wuhan Servicebio Technology
Sourced in China
The TUNEL Apoptosis Assay Kit is a laboratory product designed to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method to label and identify DNA fragmentation, which is a hallmark of apoptosis. The core function of the kit is to provide a reliable and standardized approach for the analysis of apoptosis in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamino 2 phenylindole dapi, by Merck Group (3 mentions)
Lsm 710, by Zeiss (2 mentions)
Uorescent microscope, by Olympus (2 mentions)
Lipase, by Maclin Biochemical Technology (1 mentions)
Pluronic f 127 f 127, by Merck Group (1 mentions)
Diaminobenzidine dab, by Merck Group (1 mentions)
Fluorescent microscope, by Olympus (1 mentions)
Inverted fluorescence microscope, by Zeiss (1 mentions)
12 protocols using tunel apoptosis assay kit
1
Sustained Release PCL-Based Hydrogel Formulations
2
TUNEL Assay for Apoptotic Chondrocytes
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The apoptotic chondrocytes in the cartilage sections were detected using TUNEL assay following the instruction manual of TUNEL Apoptosis Assay Kit (Servicebio, Wuhan, China) .
3
Quantifying Lung Macrophage Infiltration and PASMC Proliferation
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Lung CD68 were stained by standard immunohistochemistry protocols to quantify the degree of macrophage infiltration. Briefly, CD68 expression levels were detected in paraffin embedded lung sections using anti-CD68 rat antibody (1:1000 dilution, Abcam, USA) (27 (link)). Cells positively stained for CD68 were automatically counted with image J software (NIH, Bethesda, MD, USA), which was calculated as the mean of 5 random fields from scanned images (DSAssistantLite software, Motic, China; ×400 magnification, image size: 715 × 408 μm). And the CD68 positive rate are calculated by CD68 positive cells to total cells in each field.
The proliferative and apoptotic abilities of PASMCs were, respectively, determined by immunohistochemical staining of lung section with Ki67 antibody (cat. no. GB111141, Servicebio, China) and TUNEL apoptosis assay kit (cat. no. G1507, Servicebio, China). Immunohistochemical staining was performed using the two-step immunohistochemical technique with diaminobenzidine (DAB, Sigma-Aldrich, USA) following the manufacturer's instructions. The positive stained cells were counted by image J software (NIH, Bethesda, MD, USA), and presented as the mean of 5 randomly selected pulmonary arteriole under ×400 magnification.
The proliferative and apoptotic abilities of PASMCs were, respectively, determined by immunohistochemical staining of lung section with Ki67 antibody (cat. no. GB111141, Servicebio, China) and TUNEL apoptosis assay kit (cat. no. G1507, Servicebio, China). Immunohistochemical staining was performed using the two-step immunohistochemical technique with diaminobenzidine (DAB, Sigma-Aldrich, USA) following the manufacturer's instructions. The positive stained cells were counted by image J software (NIH, Bethesda, MD, USA), and presented as the mean of 5 randomly selected pulmonary arteriole under ×400 magnification.
4
TUNEL Assay for Apoptotic Neurons
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The apoptotic neurons in the brain sections were detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) assay. After deparaffinization and rehydration, the brain sections were permeabilized with proteinase K solution, then exposed to the mixture of biotinylated nucleotide dUTP and recombinant terminal deoxynucleotidyl transferase (TdT) following the instruction manual of TUNEL Apoptosis Assay Kit (Servicebio, Wuhan, China). Staining with 4,6-diamino-2-phenyl indole (DAPI) (Sigma, St. Louis, USA) was performed to visualize nuclei. Images were obtained under a fluorescent microscope (Olympus, Center Valley, USA).
5
Detecting Apoptotic Chondrocytes via TUNEL Assay
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The apoptotic chondrocytes in the sections were detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) assay following the instruction manual of TUNEL Apoptosis Assay Kit (Servicebio, Wuhan, China) .
6
TUNEL Assay for Apoptosis Detection
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TUNEL staining was performed using a TUNEL Apoptosis Assay Kit (G1501; Servicebio, Wuhan, China), as described previously [6 (link)]. TUNEL+ cells were counted manually under an inverted fluorescence microscope (Carl Zeiss, Oberkochen, German).
7
Apoptosis Mapping in Zebrafish Embryo Brains
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Zebrafish embryos (48 hpf) were collected, and the egg membranes were manually removed and then fixed with 4% PFA at −4 °C. Then, the heads of the embryos were cut off and soaked in the agar-sucrose mixed gel. Samples were fixed in the standard position. Agarose gel-containing samples were cut into cubes with a side length of 0.3 cm post-solidification. The gel cubes were dehydrated with 30% sucrose for 2 days. OCT embedded heads were frozen at −25 °C for 30 min prior to sectioning at 5 μm per slice and stored at −80 °C until use. Intracerebral apoptotic cells were examined by TUNEL assay. Sample slides were fixed with 4% PFA for 30 min, and permeablized by proteinase K and 0.1% Triton X-100. Following the protocols of TUNEL Apoptosis Assay Kit (Servicebio), samples were further balanced and stained with a reaction mixture and DAPI. Then they were rinsed with PBST, and sealed with quench-resistant sealing fluid. Lastly, Images were captured using a confocal microscope and analyzed using Image J software.
8
Histological Analysis of Tissues
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The lung, liver, kidney, spleen, heart, and tumor tissues were embedded in paraffin after formalin was fixed and sectioned at 5 μm thickness for further analysis. The sections were stained with H&E and visualized by fluorescence microscopy according to the operation manual. The apoptotic tumor cells were also evaluated using the TUNEL Apoptosis Assay Kit (Servicebio, China) according to the recommended instructions and examined by fluorescence microscopy (Axiscope5, Carl Zeiss, Germany). The immunohistochemistry (IHC) staining of Ki67 was examined using a Ki67-antibody Assay Kit (Servicebio, China) according to standard protocols.
9
TUNEL Assay for Apoptosis Detection
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Apoptotic cells were detected with the TUNEL Apoptosis Assay Kit (T2190-50 T, Servicebio) according to the manufacturer’s instructions. The nuclei were stained with DAPI, and images were acquired under a confocal microscope (LSM710, Zeiss, Heidenheim, Germany).
10
Detecting Apoptotic Cells via TUNEL
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Apoptotic cells were detected with the TUNEL Apoptosis Assay Kit (T2190-50 T, Servicebio) according to the manufacturer’s instructions. The nuclei were stained with DAPI, and images were acquired by a confocal microscope (LSM710, Zeiss, Heidenheim, Germany).
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