Basic drugs and their metabolites (AMP, MAMP, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), morphine, 6-acetyl-morphine, codeine, dihydrocodeine, cocaine, benzoylecgonine, cocaethylene, methadone/EDDP, MPH) were determined in hair by a validated achiral LC-MS/MS method. This method comprises chromatographic separation of the analytes on a Kinetex 2.6 μm F5 column (150 x 2.1 mm, Phenomenex, Torrance, CA, USA) using gradient elution with water/acetonitrile containing 0.1% formic acid, followed by tandem-mass spectrometric detection with a 5500 QTRAP® hybrid triple quadrupole/linear ion trap mass spectrometer and a Turbo V ion source (SCIEX, Brugg, Switzerland) operated in positive ESI and SRM mode. Data were acquired and analyzed with Analyst software version 1.6.2 (SCIEX, Brugg, Switzerland). For all analytes that were quantitatively determined, linearity ranges are from 100–5000 pg/mg and the LLOQ is 100 pg/mg. This method has been validated and is used in the accredited laboratory. Further details of this method can be obtained from the authors on request.
Kinetex 2.6 μm f5 column
Manufactured by Phenomenex
Sourced in United States
The Kinetex 2.6 μm F5 column is a laboratory equipment product designed for chromatographic separation and analysis. It features a 2.6 μm particle size and an F5 stationary phase. The core function of this column is to provide efficient and high-resolution separations in liquid chromatography applications.
Automatically generated - may contain errors
2 protocols using kinetex 2.6 μm f5 column
1
Validated Hair Quantification of Drugs
2
Comprehensive LC-DAD-MS Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-DAD-MS was performed with a AB Sciex TripleTOF 5600+ LC/MS system (AB Sciex, Framingham, USA), using a Kinetex 2.6 μm F5 column (100 mm × 2.1 mm; Phenomenex, Torrance, USA) with a flow rate of 0.2 mL/min. The mobile phase consisted of water (A, with 0.1 % formic acid) and acetonitrile (B). Gradient elution was performed as follows: 0–1 min, 95 % (v/v) A; 1–8 min, 95 % (v/v) A to 15 % (v/v) A; 8–9 min, 15 % (v/v) A; 9–9.1 min, 15 % A (v/v) to 95 % (v/v) A; 9.1–11 min, 95 % (v/v) A. Ten microliters of each sample was injected into the column. The temperatures of column and samples were held at 30 °C and 4 °C, respectively. The detection wavelength of DAD detector was from 210 to 600 nm. Mass spectrometry (MS) was performed using a triple quadrupole detector equipped with an electrospray ionization (ESI) source in positive ionization mode. The scan range was m/z 100–1000. Ionization spray voltage was set to 5500 V. Sheath gas was 379 kPa, auxiliary gas was 379 kPa, and curtain gas 172 kPa. Temperature of ESI source was 550 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!