Mouse monoclonal anti gapdh antibody sc 32233

Mouse monoclonal anti-GAPDH antibody (sc-32233), rabbit polyclonal anti-ERK1 antibody sc-94), mouse monoclonal anti-pERK antibody (sc-7383), and mouse monoclonal anti-histone H1 antibody (sc-8030) were purchased from Santa Cruz Biotechnology (Dallas, TX) and used for Western blot (WB) analysis. Anti-PGC-1α antibody (ab54481) was obtained from Abcam (Cambridge, UK) and used for WB and IHC analyses. Anti-8-OHdG antibody (MOG-020P) was obtained from the Japan Institute for the Control of Aging (Shizuoka, Japan). Rabbit polyclonal anti-p38 MAPK antibody and rabbit polyclonal anti-phospho-p38 MAPK were obtained from Cell Signaling Technology, Japan (Tokyo, Japan). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (170-6515; Bio-Rad) and HRP-conjugated donkey anti-goat IgG (sc-2020; Santa Cruz Biotechnology) were used as secondary antibodies for WB analysis.

Rabbit monoclonal anti-galactosidase alpha antibody (ab 168341; Abcam, Cambrige, UK; for Wb 1:3000; for IF 1:500); mouse monoclonal anti-GAPDH antibody (sc-32233, Santa Cruz Biotechnology, Dallas, TX, USA; for Wb 1:1000;), anti-beta-actin-peroxidase conjugated (A3854, Merck, Burlington, MA, USA; for Wb 1:100,000), mouse anti-KDEL (10C3, Enzo Life Sciences, NY, USA; for IF 1:500); mouse anti-LAMP1 (H4A3: Developmental Studies Hybridoma Bank, Dshb, Iowa, IA, USA; for IF 1:500); rabbit monoclonal anti-PERK (#3192, Cell Signaling, Danvers, MA, USA for Wb 1:1000).