ATR–FT–IR measurements were performed using a Bruker Tensor 37 FT-IR spectrometer (Ettlingen, Germany) equipped with a mercury cadmium telluride (MCT) detector (D* = 4 × 1010 cm Hz0.5 W−1 at 9.2 μm). The spectrometer was constantly flushed with dry air in order to reduce the influence of water vapor from the atmosphere. One drop of pure milk fat extract was manually placed onto a Platinum ATR single-bounce element (Bruker, Ettlingen, Germany). Measurements were performed with a spectral resolution of 2 cm−1, between 600 and 4000 cm−1 in double-sided, forward–backward acquisition mode. A Blackman–Harris 3-term apodization function and a zero-filling factor of 2 were used to calculate the final spectra. One hundred and twenty-eight scans were averaged per spectrum, leading to an acquisition time of fifty-two seconds. After each spectral acquisition, the ATR surface was cleaned with isopropanol and dichloromethane consecutively until recovery of the baseline signal. Transmission measurements were performed using the same instrument parameters, by injecting homogenized whole milk into a flow cell equipped with two CaF2 windows and a 37 µm-thick spacer. The software package OPUS 7.2 (Bruker, Ettlingen, Germany) was used for evaluation of the spectral data.
Platinum atr single bounce element
Manufactured by Bruker
Sourced in Germany
The Platinum ATR single-bounce element is a core component of Bruker's analytical equipment. It provides high-performance attenuated total reflection (ATR) capabilities for various spectroscopic applications.
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Lab products found in correlation
2 protocols using platinum atr single bounce element
1
ATR-FT-IR Spectroscopic Analysis of Milk Fat
2
FTIR Analysis of Human Milk Lipids
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A Bruker Tensor 37 FTIR spectrometer (Ettlingen, Germany) equipped with a mercury cadmium telluride (MCT) detector (D* = 4 × 10 10 cm Hz 0.5 W -1 at 9.2 μm) was used for all mid-IR measurements. Data acquisition was performed by applying the following settings: spectral resolution of 2 cm -1 , double-sided, forward-backward acquisition mode, scan range from 600 to 4000 cm -1 , 52 s measurement time (averaging 128 scans per spectrum). The final spectra were calculated by using a Blackman-Harris 3-term apodization function and a zero-filling factor of 2.
By constantly flushing the spectrometer with dry air, the influence of atmospheric water vapor was reduced. ATR measurements were performed by transferring approximately 5 µL of the extracted lipid fraction onto a Platinum ATR single-bounce element (Bruker, Ettlingen, Germany). After each measurement, the surface of the ATR element was cleaned with dichloromethane and isopropanol until the initial baseline signal was recovered. Reference measurements were performed by placing homogenized human milk into a CaF2 transmission cell with an optical path length of 50 µm (as used in the commercially available MIRIS human milk analyzer). Data was acquired and analyzed with the spectroscopy software OPUS 7.2 (Bruker, Ettlingen, Germany).
By constantly flushing the spectrometer with dry air, the influence of atmospheric water vapor was reduced. ATR measurements were performed by transferring approximately 5 µL of the extracted lipid fraction onto a Platinum ATR single-bounce element (Bruker, Ettlingen, Germany). After each measurement, the surface of the ATR element was cleaned with dichloromethane and isopropanol until the initial baseline signal was recovered. Reference measurements were performed by placing homogenized human milk into a CaF2 transmission cell with an optical path length of 50 µm (as used in the commercially available MIRIS human milk analyzer). Data was acquired and analyzed with the spectroscopy software OPUS 7.2 (Bruker, Ettlingen, Germany).
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