Rabbit anti ho 1 antibody

Manufactured by Solarbio

Sourced in China

Rabbit anti-HO-1 antibody is a primary antibody that specifically recognizes the heme oxygenase-1 (HO-1) protein. HO-1 is an enzyme involved in the degradation of heme, a process that plays a crucial role in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to detect and analyze the expression of HO-1 in biological samples.

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Lab products found in correlation

Rabbit anti nrf2 antibody, by Proteintech (2 mentions) Rabbit anti nox4 antibody, by Proteintech (2 mentions) Bx40 upright light microscope, by Olympus (1 mentions) Histone simple stain kit, by Nichirei Biosciences (1 mentions) Rabbit anti collagen 3 antibody, by Solarbio (1 mentions) Rabbit anti bax antibody, by Proteintech (1 mentions) Rabbit anti bcl 2 antibody, by Proteintech (1 mentions)

2 protocols using rabbit anti ho 1 antibody

Immunohistochemical Analysis of Cellular Markers

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Immunohistochemical analysis was performed using the HistoneSimple stain kit (Nichirei, Tokyo, Japan), according to the manufacturer’s instructions. Paraffin-embedded sections were deparaffinised with xylene and then rehydrated in a descending series of ethanol washes. The sections were treated for 15 min with 3% H₂O₂ in methanol to inactivate endogenous peroxidases and then incubated at 4 °C overnight with primary antibodies to SIRT1 (rabbit anti-SIRT1 antibody, 1:300; Solarbio), NRF2 (rabbit anti-NRF2 antibody, 1:200; Proteintech), NOX4 (rabbit anti-NOX4 antibody, 1:200; Proteintech), TGF-β (rabbit anti-TGF-β antibody, 1:200; Solarbio), HO-1 (rabbit anti-HO-1 antibody, 1:100; Solarbio), collagen III (rabbit anti-collagen III antibody, 1:100; Solarbio), Smad3 (rabbit anti-Smad3 antibody, 1:100; Solarbio), Bax (rabbit anti-Bax antibody, 1:50; Solarbio), Bak (rabbit anti-Bak antibody, 1:100; Solarbio), Bcl-2 (rabbit anti-Bcl-2 antibody, 1:50; Solarbio) and Bcl-xl (rabbit anti-Bcl-xl antibody, 1:100; Solarbio). All sections were examined microscopically using a BX40 upright lightmicroscope (Olympus, Tokyo, Japan).

Pei Z., Ji J., Gao Y., Wang H., Wu Y., Yang J., Yang Q, & Zhang L. (2023). Exercise reduces hyperlipidemia-induced cardiac damage in apolipoprotein E-deficient mice via its effects against inflammation and oxidative stress. Scientific Reports, 13, 9134.

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Immunohistochemical Analysis of Cardiac Markers

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Paraffin-embedded cardiac tissues were cut into 5-μm-thick cross-sections and
deparaffinized prior to staining using a standard protocol. Immunohistochemical
staining was performed according to the manufacturer's instructions (Zsbio,
Beijing, China) using antibodies against Bax (rabbit anti-Bax antibody, 1:200;
Proteintech, Wuhan, China), Bcl-2 (rabbit anti-Bcl-2 antibody, 1:200;
Proteintech), NRF2 (rabbit anti-NRF2 antibody, 1:200; Proteintech), NOX4 (rabbit
anti-NOX4 antibody, 1:200; Proteintech), and HO-1 (rabbit anti-HO-1 antibody,
1:200; SOLARBIO, Beijing, China). All sections were examined using a BX40
upright light microscope.

Guo W., Long X., Lv M., Deng S., Liu D, & Yang Q. (2022). Effect of thymoquinone on sepsis-induced cardiac damage via anti-inflammatory and anti-apoptotic mechanisms. The Journal of International Medical Research, 50(9), 03000605221118680.

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