The PFC samples of rats were homogenized, and proteins were extracted using a Tris–EDTA lysis buffer (1% Triton X-100, 10% glycerol, 20 mM Tris, pH 7.5, 1 mM EDTA) containing freshly added protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). After protein determination using a BCA kit, sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed. The antibody against β-tubulin (1:1000) (Sigma-Aldrich, St. Louis, MO, USA) was used as an internal control for the concentration of protein loaded, while the primary antibodies against ATP-α (1:1000), GLT-1 (1:5000), GLAST (1:2000), GAD65 (1:1000), GS (1:500), or PAG (1:1000) were used to detect the corresponding target proteins. After incubation in a secondary antibody solution (anti-rabbit antibody, 1:1000), the immunoreactive bands were visualized using an ECL detection kit (Amersham Biosciences, Buckinghamshire, UK). Image Lab software version 5.0 (Bio-Rad) was used for image acquisition and densitometric analysis of the immunoreactive bands. The data were expressed as folds of α-tubulin band in a corresponding lane.
Antibody against β tubulin
Manufactured by Merck Group
Sourced in United States
The Antibody against β-tubulin is a laboratory reagent used to detect and study the β-tubulin protein, a key component of the cytoskeleton in eukaryotic cells. This antibody can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to visualize and quantify the presence and distribution of β-tubulin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Image lab software version 5, by Bio-Rad (2 mentions)
Ecl detection kit, by Cytiva (2 mentions)
Protease inhibitor cocktail, by Merck Group (2 mentions)
Lysotracker deep red, by Thermo Fisher Scientific (2 mentions)
Dylight 488 nhs ester, by Thermo Fisher Scientific (2 mentions)
Sulforhodamine b, by Merck Group (2 mentions)
Trastuzumab, by Roche (2 mentions)
T dm1, by Roche (2 mentions)
Anti pgrnfl, by R&D Systems (1 mentions)
Phospho stat1 tyr701, by Cell Signaling Technology (1 mentions)
Phospho stat5 tyr694, by Cell Signaling Technology (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Imatinib, by Selleck Chemicals (1 mentions)
Sunitinib, by Selleck Chemicals (1 mentions)
Dasatinib, by Selleck Chemicals (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Gdc 0941, by Selleck Chemicals (1 mentions)
6 protocols using antibody against β tubulin
1
Western Blot Analysis of Neuronal Proteins
2
Quantifying Protein Levels in Cell Lysates
3
Antibody-drug Conjugate T-DM1 Internalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The T‐DM1 and trastuzumab were purchased from F. Hoffmann‐La Roche (Basel, Switzerland). Napabucasin and DM1 were purchased from Meilunbio Inc. (Dalian, China). LysoTracker Deep Red and DyLight 488 NHS ester were purchased from Thermo‐Fisher Scientific (Waltham, MA, USA). Propidium iodide, sulforhodamine B, and the antibody against β‐tubulin were purchased from Sigma‐Aldrich (St Louis, MO, USA). Antibodies against HER2, P‐glycoprotein (P‐gp), phospho‐STAT1 (Tyr701), STAT1, phospho‐STAT3 (Tyr705), STAT3, phospho‐STAT5 (Tyr694), STAT5, phospho‐EGFR (Tyr845), phospho‐HER3 (Tyr1289), phospho‐c‐MET (Tyr1234/1235), phospho‐FGFR1 (Tyr653/654), phospho‐histone H3 (Ser10), PARP and c‐Myc were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against leukemia inhibitory factor receptor (LIFR), interleukin‐6 receptor (IL‐6R), granulocyte‐macrophage colony‐stimulating factor receptor (GM‐CSFR), β‐actin, and caspase‐3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
4
Signaling Pathway Inhibitors in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T-DM1 and trastuzumab were purchased from F. Hoffmann-La Roche (Basel, Switzerland). Gefitinib, AZD4547, crizotinib, sunitinib, imatinib, dasatinib, PD 0325901 and GDC-0941 were purchased from Selleck Chemicals (Houston, TX, USA). DM1 was purchased from Meilunbio Inc. (Dalian, China). T-DM1 and trastuzumab were dissolved in saline, and small molecule compounds were dissolved in dimethyl sulfoxide. Lyso-Tracker Deep Red and DyLight 488 NHS ester were purchased from Thermo-Fisher Scientific (Waltham, MA, USA). Sulforhodamine B and the antibody against β-tubulin were purchased from Sigma–Aldrich (St. Louis, MO, USA). Antibodies against phospho-HER2 (Tyr1221/1222), HER2, phospho-EGFR (Tyr845), EGFR, phospho-HER3 (Tyr1289), HER3, phospho-Met (Tyr1234/1235), phospho-IGF-IR (Tyr1135/1136)/IR (Tyr1150/1151), phospho-Akt (Ser473), phospho-Erk1/2 (Thr202/Tyr204), phospho-PTEN (Ser380/Thr382/383), PTEN, phospho-Src family kinase (Tyr416), c-Src, Yes, Fyn, Lyn, Lck, Csk and PARP were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Erk1/2, p27, TYMS, and THOC1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
5
Protein Quantification and Western Blotting in Rat PFC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PFC samples of rats were homogenized, and proteins were extracted using a
Tris-EDTA lysis buffer (1% Triton X-100, 10% glycerol, 20 mM Tris, pH 7.5, 1 mM EDTA) containing freshly added protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). After protein determination using a BCA kit, sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Western blotting were performed. The antibody against β-tubulin (1:1000) (Sigma-Aldrich, St. Louis, MO, USA) was used as an internal control for the concentration of protein loaded, while the primary antibodies against ATP-α (1:1000), GLT-1 (1:5000), GLAST (1:2000), GAD65 (1:1000), GS (1:500), or PAG (1:1000) were used to detect the corresponding target proteins. After incubation in a secondary antibody solution (anti-rabbit antibody, 1:1000), the immunoreactive bands were visualized using an ECL detection kit (Amersham Biosciences, Buckinghamshire, UK). Image Lab software version 5.0 (Bio-Rad) was used for image acquisition and densitometric analysis of the immunoreactive bands. The data were expressed as folds of α-tubulin band in a corresponding lane.
Tris-EDTA lysis buffer (1% Triton X-100, 10% glycerol, 20 mM Tris, pH 7.5, 1 mM EDTA) containing freshly added protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). After protein determination using a BCA kit, sodium dodecyl sulfatepolyacrylamide gel electrophoresis and Western blotting were performed. The antibody against β-tubulin (1:1000) (Sigma-Aldrich, St. Louis, MO, USA) was used as an internal control for the concentration of protein loaded, while the primary antibodies against ATP-α (1:1000), GLT-1 (1:5000), GLAST (1:2000), GAD65 (1:1000), GS (1:500), or PAG (1:1000) were used to detect the corresponding target proteins. After incubation in a secondary antibody solution (anti-rabbit antibody, 1:1000), the immunoreactive bands were visualized using an ECL detection kit (Amersham Biosciences, Buckinghamshire, UK). Image Lab software version 5.0 (Bio-Rad) was used for image acquisition and densitometric analysis of the immunoreactive bands. The data were expressed as folds of α-tubulin band in a corresponding lane.
6
Mortalin Silencing Impacts Autophagy
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!