Nu1025

Inhibitors (PARP inhibitor (Pi) 100 μM NU1025 (Sigma) or 20 μM olaparib (Apexbio Technology), 10 μM DNA–PKcs inhibitor (Di) NU7026 (Sigma), 10 μM ATM inhibitor (Ai) KU55933 (Calbiochem), 1 μM PARG inhibitor (PARGi) DEA ((6,9-diamino-2-ethoxyacridine lactate monohydrate) (Trevigen)) were added to the cell culture one hour prior to damage induction. DMSO only was added to control cells.

The c‐MET inhibitor SU11274 (#S9820) and PARP inhibitor NU1025 (#N7287) were obtained from Sigma‐Aldrich. Both inhibitors were dissolved in DMSO and stored at − 80°C.

Source of cell lines used in the study is reported in the reagent and resource table. Rtel1^f/f mice (described in (Wu et al., 2007 (link))) were crossed with early generation Terc^+/− mice (described in (Lee et al., 2001 (link))). All mice were housed and maintained according to the Home Office guidance outlined in the Animal (Scientific Procedures) Act 1986. Mouse adult ear fibroblasts (MAFs) cell lines were derived from male aged matched Rtel1^f/fTerc^+/+ and Rtel1^f/fTerc^−/− sibling mice. SV40-LT-immortalized as well as primary Rtel1^f/fTerc^+/+ and Rtel1^f/fTerc^−/− MAFs, SV40-LT-immortalized Rtel1^f/f (Vannier et al., 2012 (link)) and Trf2^f/- (Celli and de Lange, 2005 (link)) a gift from Titia de Lange, The Rockefeller University) MEFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (Invitrogen), L-glutamine, and penicillin-streptomycin. Deletion of floxed alleles in Rtel1^f/f and Trf2^f/- cells was carried out with either Ad-GFP or Ad-GFP-Cre adenovirus (Vector Biolabs). Cells were genotyped by PCR at 96 hr post-infection to confirm gene deletion. Olaparib (Selleckchem) and NU1025 (Sigma) were used at 5μM and 10μM, respectively, for 48 hours prior to cell collection. GRN163L (a gift from Jerry Shay, UT Southwestern) and BIBR1532 (Santa Cruz) were used at 2μM and 10μM, respectively, for 48 hours prior to cell collection.