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Hif 1α nb100 134

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HIF-1α (NB100-134) is a primary antibody targeting the Hypoxia-Inducible Factor 1-alpha (HIF-1α) protein. HIF-1α is a subunit of the HIF-1 transcription factor, which plays a crucial role in the cellular response to hypoxia.

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Lab products found in correlation

Bcl 6 c 19, by Santa Cruz Biotechnology (2 mentions) C myc n 262, by Santa Cruz Biotechnology (2 mentions) H3k9ac, by Abcam (2 mentions) Gabpa sc 22810x, by Santa Cruz Biotechnology (2 mentions) Magna chip a g, by Merck Group (2 mentions) Creb1, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

HIF-1α (152 citations) NB100-134 (24 citations) HIF-1α antibody (NB100-134, (2 citations)

4 protocols using hif 1α nb100 134

1

Profiling Chromatin Modifications in T Helper Cells

Cited 2 times
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The ChIP assay was performed as previously published9 (link),49 (link),50 (link). Chromatin from primary polarized CD4+ TH1 cells maintained in variable IL-2 conditions was precipitated with antibodies to either Bcl-6 (C-19; Santa Cruz Biotechnology), c-Myc (N-262; Santa Cruz Biotechnology), HIF-1α (NB100-134; Novus Biologicals), or H3K9Ac (AB4441; Abcam). The purified DNA was analyzed by qPCR with the indicated primers. The experimental samples were first normalized to a standardized input DNA control then the IgG antibody control was subtracted to account for the nonspecific background, with this value graphically represented as the percent input of each sample.
Oestreich K.J., Read K.A., Gilbertson S.E., Hough K.P., McDonald P.W., Krishnamoorthy V, & Weinmann A.S. (2014). Bcl-6 directly represses the gene program of the glycolysis pathway. Nature immunology, 15(10), 957-964.
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2

Profiling Chromatin Modifications in T Helper Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The ChIP assay was performed as previously published9 (link),49 (link),50 (link). Chromatin from primary polarized CD4+ TH1 cells maintained in variable IL-2 conditions was precipitated with antibodies to either Bcl-6 (C-19; Santa Cruz Biotechnology), c-Myc (N-262; Santa Cruz Biotechnology), HIF-1α (NB100-134; Novus Biologicals), or H3K9Ac (AB4441; Abcam). The purified DNA was analyzed by qPCR with the indicated primers. The experimental samples were first normalized to a standardized input DNA control then the IgG antibody control was subtracted to account for the nonspecific background, with this value graphically represented as the percent input of each sample.
Oestreich K.J., Read K.A., Gilbertson S.E., Hough K.P., McDonald P.W., Krishnamoorthy V, & Weinmann A.S. (2014). Bcl-6 directly represses the gene program of the glycolysis pathway. Nature immunology, 15(10), 957-964.
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3

Chromatin Immunoprecipitation Assay Protocol

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ChIP assays were performed using 500 micrograms of chromatin. Chromatin was sonicated to fragments of ~500 bp and immunoprecipitated using 1 microgram of ChIP-grade antibodies: HIF1α (NB100–134, Novus Biological), GABPA (sc-22810X, Santa Cruz Biotechnology ), CREB1 (9104, Cell Signaling Technology) and with irrelevant IgG antibodies of mouse and rabbit origin and recovered using protein A/G magnetic beads (from Magna ChIP™ A/G, Millipore). The precipitated DNA was amplified by real-time qPCR, using primer sets designed to amplify regions of the target genes. ChIP primer sequences are provided in Supplementary Table 3:
Chhipa R.R., Fan Q., Anderson J., Muraleedharan R., Huang Y., Ciraolo G., Chen X., Waclaw R., Chow L.M., Khuchua Z., Kofron M., Weirauch M.T., Kendler A., McPherson C., Ratner N., Nakano I., Dasgupta N., Komurov K, & Dasgupta B. (2018). AMP Kinase Promotes Glioblastoma Bioenergetics and Tumor Growth. Nature cell biology, 20(7), 823-835.
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4

Chromatin Immunoprecipitation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed using 500 micrograms of chromatin. Chromatin was sonicated to fragments of ~500 bp and immunoprecipitated using 1 microgram of ChIP-grade antibodies: HIF1α (NB100–134, Novus Biological), GABPA (sc-22810X, Santa Cruz Biotechnology ), CREB1 (9104, Cell Signaling Technology) and with irrelevant IgG antibodies of mouse and rabbit origin and recovered using protein A/G magnetic beads (from Magna ChIP™ A/G, Millipore). The precipitated DNA was amplified by real-time qPCR, using primer sets designed to amplify regions of the target genes. ChIP primer sequences are provided in Supplementary Table 3:
Chhipa R.R., Fan Q., Anderson J., Muraleedharan R., Huang Y., Ciraolo G., Chen X., Waclaw R., Chow L.M., Khuchua Z., Kofron M., Weirauch M.T., Kendler A., McPherson C., Ratner N., Nakano I., Dasgupta N., Komurov K, & Dasgupta B. (2018). AMP Kinase Promotes Glioblastoma Bioenergetics and Tumor Growth. Nature cell biology, 20(7), 823-835.
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