Norethindrone acetate

Medroxyprogesterone acetate (MPA), Norethindrone Acetate (NET), Levonorgestrel (LNG), or progesterone (P) (All Sigma-Aldrich, St. Louis, MO) were dissolved in 100% ethanol for an initial concentration of 1000 µM, evaporated to dryness in a glass scintillation vial and resuspended to a concentration of 10 µM in stripped media. Stripped media was DMEM/F12 supplemented with charcoal–dextran stripped FBS (Gemini Bio-Products, West Sacramento, CA), Penicillin–Streptomycin (Thermo Fisher), l-Glutamine (Thermo Fisher), and HEPES (GE Life Sciences). Further dilutions were made to achieve final working concentrations of 0.1 to 100 nM. As a control, an equivalent amount of ethanol without hormone was evaporated to dryness prior to media addition.

17β-estradiol (E2, #E2758); progesterone (P4, #P0130); levonorgestrel (LNG, #1362602); medroxyprogesterone acetate (MPA, #1378001); nestorone (NES, # SML0550); norethindrone (NET, #1469005); norethindrone acetate (NETA, #1470004); norgestimate (NGM, # 1471914); hydroxyprogesterone caproate (OHPC, #1329006), and resveratrol (RSV, #R5010) were obtained from Sigma. Norethynodrel (NEN, #E4600–000) was obtained from Steraloids.