Model 490 4 four channel optical aggregometer

Manufactured by Chrono-Log

The Model 490 4 + Four Channel Optical Aggregometer is a laboratory instrument used to measure the aggregation of platelets in whole blood or platelet-rich plasma samples. It utilizes optical detection methods to monitor and record the changes in light transmission through the sample over time, which is a reliable indicator of platelet aggregation.

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Rabbit igg isotype control antibody, by Abcam (1 mentions)

Close spelling variants of this product

4‐channel optical aggregometer Model 490 aggregometer Chrono-Log 490 aggregometer Optical aggregometer Model 490 Aggregometer

2 protocols using model 490 4 four channel optical aggregometer

Platelet Aggregation Assay

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Aggregation was tested using a Model 490 4 + Four Channel Optical Aggregometer (Chronolog, Havertown, PA) according to manufacturer’s protocol. Platelet rich plasma (PRP) was collected from whole blood by centrifugation at 170× g for 15 min. Platelet poor plasma was collected from whole blood by centrifugation at 1500× g for 15 min. Platelets were stimulated with collagen (2 μg/ml), ADP (10 μM), epinephrine (10 μM), or thrombin (0.5 units/ml) for 10 min and maximum aggregation was measured.

Garofano K., Rashid K., Smith M., Brantner C., Suwunnakorn S., Diemert D., Gordon O., Horvath A., Khan S., Popratiloff A., Rhim J., Sidahmed A., Maggirwar S.B., O’Brien T.J., Perera M.A, & Lee N.H. (2023). Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs. Scientific Reports, 13, 2864.

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Platelet Aggregation Modulation by IL32 and PROK2

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Platelet rich plasma (PRP) was collected from whole blood via centrifugation at 170 × g for 15 minutes. Platelets were stimulated using collagen (2 μg/ml), ADP (10 μM), epinephrine (10 μM), and thrombin (0.5 units/ml) for 10 minutes. Aggregation was performed using light transmission aggregometry on a Model 490 4+ Four Channel Optical Aggregometer (Chrono-log). Maximum aggregation, rate of aggregation, and time until start of aggregation were all recorded following stimulation.
To test the effect of neutralizing antibodies, PRP was incubated with an anti-IL32 (Abcam, 5 ug/mL), anti-PROK2 (Invitrogen, 5 ug/mL), or a rabbit IgG isotype control antibody (Abcam, 5 ug/mL) for five minutes. Aggregation was then stimulated with collagen (2 μg/ml).
Washed platelets were isolated from whole blood samples as previously described (27 (link)), and aggregation assays were performed by incubating with 400 ug/mL fibrinogen and either IL32 (500 nM, Sigma) or PROK2 (10 nM, Sigma) prior to stimulation with collagen (2 μg/ml).

Garofano K., Park C.S., Alarcon C., Avitia J., Barbour A., Diemert D., Fraser C.M., Friedman P.N., Horvath A., Rashid K., Shaazuddin M., Sidahmed A., O’Brien T.J., Perera M.A, & Lee N.H. (2021). Differences in the platelet mRNA landscape portend racial disparities in platelet function and suggest novel therapeutic targets. Clinical pharmacology and therapeutics, 110(3), 702-713.

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