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Dmlm confocal microscope

Manufactured by Leica
Sourced in United Kingdom

The DMLM confocal microscope is a laboratory imaging instrument designed for high-resolution observation and analysis of microscopic samples. The core function of the DMLM is to capture detailed, three-dimensional images of specimens by utilizing a confocal scanning system that eliminates out-of-focus light, enabling the user to focus on specific planes within a sample.

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2 protocols using dmlm confocal microscope

1

Raman Spectroscopy of Carbon Samples

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Raman spectra were recorded at room temperature by an InVia spectrometer (Renishaw, Wotton-under-Edge, UK) equipped with a DMLM confocal microscope (Leica, Wetzlar, Germany) and CCD detector. The lasers with the excitation wavelengths of 514 and 785 nm were used. The measurements were carried out with the resolution of 2 cm−1, in the range of 100–1800 cm−1. The Fityk software was used to deconvolute Raman spectra of carbons samples [41 (link)].
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2

Micro-Raman Spectroscopy of Samples

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The micro-Raman
spectra were acquired by using a Renishaw InVia spectrometer coupled
to a Leica DMLM confocal microscope. The excitation wavelength was
514.5 nm, and the objective magnification was 50x. Ten scans were
taken for each sample with a resolution of 1 cm–1 to obtain sufficient signal-to-noise. The spectra were collected
in the spectral range of 1000–3000 cm–1.
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