Anti nlrc4

The following antibodies were used: monoclonal anti-TUBB5 (Sigma, T8328); anti-CASP1/caspase 1 p10 (Santa Cruz Biotechnology, sc-514), anti-8-OHdG (Santa Cruz Biotechnology, sc-66036); anti-IL1B (Abcam, ab13810); anti-LC3B (Novus Biological, NB100–2220), anti-NLRC4 (Novus Biological, NBP1–78979ss), anti-PINK1 (Novus Biological, BC100–494); anti-BrdU (Sigma, Bu33); anti-dsDNA (abcam, 27156); anti-rabbit IgG IP beads (eBioscience, 00–880025), anti-ATG7 (Cell Signaling Technology, 2631s); anti-ATG5 (Novus Biological, NB110–53818ss). Bound antibody was visualized using anti-rabbit (Cell Signaling Technology, 7074s) or anti-mouse (Cell Signaling Technology, 7076) horseradish peroxidase-coupled immunoglobulin and chemiluminescence ECL kit (GE healthcare, RPN2209).

Anti‐GSDMD (Cat. no. NBP2‐33422), anti‐NLRC4 (Cat. no. NB100‐56142), anti‐CD45 (Cat. no. NB100‐77417) and anti‐fibronectin (Cat. no. NBP1‐91258) were obtained from Novus (Centennial, CO, USA). The anti‐caspase‐1 polyclonal antibody (Cat. no. ab1872) was obtained from Abcam (Cambridge, MA, USA). Anti‐IL‐1β (Cat. no. 12242) and anti‐caspase‐5 (Cat. no. 46680) were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti‐caspase‐4 (Cat. no. 11856‐1‐AP), anti‐collagen type I (Cat. no. 14695‐1‐AP) and anti‐β‐actin (Cat. no. 60008‐1‐Ig) were obtained from Proteintech (Wuhan, China). Alexa Fluor 488–conjugated secondary antibody (Cat. no. ab150089) was obtained from Abcam. VX‐765 (Cat. no. HY‐13205) and Val‐boroPro (Cat. no. HY‐13233A) were obtained from MedChemExpress (Monmouth Junction, NJ, USA). Etoposide (Cat. no. A1971) was obtained from ApexBio (Houston, TX, USA). Streptozocin (Cat. no. S0130) was obtained from Sigma Aldrich (St Louis, MO, USA).