Blood samples for the determination of TSH, fT4, fT3, and SHBG serum concentrations and lipid profiles were collected 8 weeks after liquid levothyroxine treatment. Hormone level measurements were performed using chemiluminescence immunoassays (CLIA) using an automated analyzer for serum concentrations of TSH, fT4, fT3, and SHBG (Advia Centaur XP Immunoassay System; Siemens, Germany). The reference ranges of hormone serum concentration were set as follows: TSH- 0.38–4.0 uIU/mL; fT4- 10,29–21,88 pmol/L; fT3- 1.71–3.71 pg/mL. The reference range for SHBG depends on age and gender. Anti-thyroid peroxidase antibodies (TPO-Ab) and antithyroglobulin antibodies (Tg-Ab) were determined in all the samples by using the CLIA method (Advia Centaur XP Immunoassay System; Siemens, Germany); antibody titers were positive above 60 IU/mL. The lipid profiles, including the levels of total cholesterol, HDL-C, LDL-C and triglycerides, were measured with a Cobas 6000 biochemistry analyzer (Roche Diagnostic, Basel, Switzerland).
Cobas 6000 biochemistry analyzer
Manufactured by Roche
The Cobas 6000 is a fully automated biochemistry analyzer designed for clinical laboratories. It performs a range of in vitro diagnostic tests for the quantitative determination of analytes in various biological samples. The Cobas 6000 is capable of processing multiple sample types and can handle a high volume of tests efficiently.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cobas 6000 biochemistry analyzer
1
Thyroid Hormone and Lipid Biomarkers
2
Insulin Therapy Effect on Apolipoproteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The measurement of apo AI and apo AII concentration was conducted at 4 time points: at the time of diagnosis (before administration of insulin) and at follow-up after 3 weeks, 6 months and 1 year of insulin treatment. The concentration of apo AI and apo AII was determined with AssayPro company tests using the immunoenzymatic method ELISA (enzyme-linked immunosorbent assay) using a TECAN-SUNRISE reader with Magellan software. Apolipoprotein AI/apo AII ratio was calculated by dividing apo AI by apo AII. The evaluation of the lipid profile was conducted at the time of diagnosis (before administration of insulin). High-density lipoprotein cholesterol, total cholesterol (TC), and triglyceride (TG) levels were measured with the Cobas 6000 biochemistry analyzer (Roche Diagnostics) using enzymatic colorimetric methods. All of the above measurements were performed in serum. Low-density lipoprotein cholesterol level was calculated by the Friedewald formula. Non-HDL-C was calculated as TC minus HDL-C. Triglyceride/HDL-C ratio was calculated by dividing TG by HDL-C (in mg/dl units). To properly assess the effect of insulin therapy on apos, the inclusion criterion was to obtain baseline blood samples of the patients immediately after the diagnosis of diabetes and before administration of the first insulin injection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!