Cells were starved for 72 hours in serum-free media and then released into complete media with 5μM GW8510 (sc-215122, Santa Cruz) or the solvent DMSO for 24 hours.
Gw8510
Manufactured by Santa Cruz Biotechnology
Sourced in Canada
GW8510 is a small molecule compound that acts as a selective inhibitor of the cyclin-dependent kinase 5 (CDK5) enzyme. CDK5 is a serine/threonine protein kinase that plays a crucial role in various cellular processes, including neuronal function, cell cycle regulation, and cytoskeletal dynamics. GW8510 is primarily used in research applications to study the biological functions and signaling pathways associated with CDK5.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gw8510
1
Serum Starvation and GW8510 Treatment
2
Anticancer Drug Screening in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human colon cancer cell lines (HCT116, RKO, SW403, and SW620), and breast cancer cell lines (MCF7) were obtained from ATCC (American Type Culture Collection, Manassas, VA) and maintained as suggested by ATCC. The growth media for all cell lines were supplemented with 10% fetal bovine serum (FBS), 50 units/ml of penicillin and streptomycin, and incubated at 37°C with 5% carbon dioxide. In experiments, cells were treated with ethanol, water or DMSO as corresponding vehicle control. Phenoxybenzamine, GW-8510, etacrynic acid, ginkgolide A, triflusal, imipenem, 6-azathymine were purchased from Santa cruz (CA). Phthalylsulfathiazole was purchased from Sigma (St. Louis, MO). Phenoxybenzamine, phthalylsulfathiazole, etacrynic acid, ginkgolide were dissolved in ethanol. Imipenem was dissolved in water. The remaining drugs were dissolved in Dimethyl sulfoxide (DMSO).
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