Cy 3 anti rabbit secondary antibody

Manufactured by Jackson ImmunoResearch

Sourced in United States

Cy™−3 anti-rabbit secondary antibody is a fluorescently-labeled antibody that specifically binds to rabbit primary antibodies. It is designed for use in immunoassays and other applications that require detection of rabbit-derived proteins or antigens.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Ki67 antibody, by Abcam (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Anti s100β cell monoclonal antibody, by Cosmo Bio (1 mentions) Anti neurofilament rabbit polyclonal antibody, by Merck Group (1 mentions) Anti bdnf rabbit polyclonal antibody, by Santa Cruz Biotechnology (1 mentions) Eclipse 50i, by Nikon (1 mentions) Fitc anti mouse cd11c, by BioLegend (1 mentions) Anti luciferase, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-rabbit secondary antibody (44 citations)

4 protocols using cy 3 anti rabbit secondary antibody

Quantifying Neuroblastoma Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neuroblastoma cells were plated into 96-well plates and transfected as described above. After 120 hours, cells were fixed in 4% paraformaldehyde (Sigma-Aldrich) for 15 minutes at room temperature. Cells were permeabilized and blocked with blocking buffer (1X PBS/5% BSA/0.3% Triton™ X-100 (Sigma-Aldrich)) for 1 hour at room temperature. Cells were then incubated with Ki67 antibody (Abcam: ab15580) overnight at a dilution of 1:150. Cells were then washed three times with PBS and incubated with a Cy™−3 anti-rabbit secondary antibody (Jackson ImmunoResearch: 711-165-152) at a concentration of 1:500 for 1 hour at room temperature. Following three washes with PBS, cells were incubated in 2 μg/mL DAPI (Thermo Fisher) for 5 mins at room temperature. Cells were then manually counted utilizing an epifluorescence microscope. For each replicate, 500 cells were counted, and Ki67 positive cells were determined as the number of positive cells divided by the total number of cells (DAPI positive).

Kosti A., Du L., Shivram H., Qiao M., Burns S., Garcia J.G., Pertsemlidis A., Iyer V.R., Kokovay E, & Penalva L.O. (2019). ELF4 is a target of miR-124 and promotes neuroblastoma proliferation and undifferentiated state. Molecular cancer research : MCR, 18(1), 68-78.

+ Open protocol

+ Expand

Immunofluorescence Staining of pS6

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin sections were deparaffinized and rehydrated, and antigen retrieval was performed using 10 mM citrate buffer (pH 6.0). The sections were stained with rabbit anti-pS6 (5364S, Cell Signaling Technology, 1:250) antibody followed by Cy3 Anti-rabbit secondary antibody (111-165-144, Jackson ImmunoResearch Laboratories). Cell nuclei were visualized with DAPI staining. Digital images were obtained with a Nikon A1R confocal microscope, and fluorescence intensity was quantified using the ImageJ software (NIH).

Kogot-Levin A., Riahi Y., Abramovich I., Mosenzon O., Agranovich B., Kadosh L., Ben-Haroush Schyr R., Kleiman D., Hinden L., Cerasi E., Ben-Zvi D., Bernal-Mizrachi E., Tam J., Gottlieb E, & Leibowitz G. (2023). Mapping the metabolic reprogramming induced by sodium-glucose cotransporter 2 inhibition. JCI Insight, 8(7), e164296.

+ Open protocol

+ Expand

Immunofluorescence Staining of Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescence, the sections were washed 3 times with PBS, and then exposed to 0.1% bovine serum albumin solution containing 0.1% Tween 20 in PBS for 4 hr at room temperature. Next, sections were incubated overnight with a solution containing primary antibodies as follows: antiglial fibrillary acidic protein monoclonal antibody (1:400, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for astrocyte, anti-neurofilament rabbit polyclonal antibody (1:400, Sigma Chemical Co., St. Louis, MO, USA) for axons, anti-S100β cell monoclonal antibody (1:400, Cosmo Bio Co., Tokyo, Japan) for Schwann cells, and anti-BDNF rabbit polyclonal antibody (1:400, Santa Cruz Biotechnology) for BDNF. After washing, the sections were incubated 2 hr with secondary antibodies as follows: fluorescein isothiocyanate anti-mouse secondary antibody (1:400, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for neurofilament and Schwann cells or CY3 anti-rabbit secondary antibody (1:800, Jackson ImmunoResearch Laboratories) for astrocyte and BDNF. The sections were mounted and examined by a fluorescence microscope (Nikon Eclipse 50i, Nikon Inc., Melville, NY, USA).

Jung S.Y., Seo T.B, & Kim D.Y. (2016). Treadmill exercise facilitates recovery of locomotor function through axonal regeneration following spinal cord injury in rats. Journal of Exercise Rehabilitation, 12(4), 284-292.

+ Open protocol

+ Expand

Tracking Nanoparticle Fate in Pleural Mesothelioma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice with an established LLC-Luc MPE model were intrapleurally injected with LNPs labelled with DiR and longitudinally monitored at 1, 3, 24 and 48 h post-injection using the IVIS Lumina system. Randomly selected animals were killed at 24 and 48 h (n = 3 per time point) and major organs were dissected and imaged ex vivo by IVIS. MPE cells were collected and costained with anti-mouse CD11c-FITC (1:100 dilution; BioLegend, clone N418) and anti-luciferase (1:800 dilution; Sigma-Aldrich, catalogue no. L0159) followed by cy3-anti-rabbit secondary antibody (1:1,000 dilution; Jackson ImmunoResearch) and observed using a fluorescence microscope. DiR signals were recorded and merged with the CD11c image and the luciferase-stained image of the same field. Isolated pleural tumours were also preserved and sectioned for immunofluorescence microscopy as described above. DiR⁺ cells in MPE, pleural tumours and DLNs were also assessed by flow cytometry. To quantify the tissue concentrations of LNPs, LLC-Luc mice with MPE were killed at 1, 2, 8, 24, 48 and 72 h after intrapleural injection of Rhod-b-labelled LNPs (n = 3 per time point). Major organs and blood were collected for HPLC analyses.

Liu Y., Wang L., Song Q., Ali M., Crowe W.N., Kucera G.L., Hawkins G.A., Soker S., Thomas K.W., Miller L.D., Lu Y., Bellinger C.R., Zhang W., Habib A.A., Petty W.J, & Zhao D. (2021). Intrapleural nano-immunotherapy promotes innate and adaptive immune responses to enhance anti-PD-L1 therapy for malignant pleural effusion. Nature nanotechnology, 17(2), 206-216.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!