Occludin antibody

Manufactured by Proteintech

Sourced in China

The Occludin antibody is a protein-specific antibody used for the detection and analysis of the occludin protein in various biological samples. Occludin is a tight junction protein that plays a crucial role in the regulation of paracellular permeability and the maintenance of cell-cell adhesion.

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Lab products found in correlation

Zo 1 antibody, by Proteintech (2 mentions) Tnf α elisa kit, by Boster Bio (1 mentions) Dextran sulfate sodium (dss), by Meilun (1 mentions) Gadph antibody, by Abcam (1 mentions) Fixable viability stain 780, by BD (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Bicinchoninic acid bca protein assay kit, by Solarbio (1 mentions) Nrf2 antibody, by Affinity Biosciences (1 mentions) Chromatographic grade methanol, by Merck Group (1 mentions) Claudin 1 antibody, by Affinity Biosciences (1 mentions) Acetonitrile, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions) Myeloperoxidase mpo assay kit, by Nanjing Jiancheng (1 mentions) Phosphatase inhibitor cocktail, by CWBIO (1 mentions) Protease inhibitor cocktail, by CWBIO (1 mentions) Chamq universal sybr qrt pcr master mix, by Vazyme (1 mentions) Claudin1 antibody, by Proteintech (1 mentions) Hrp conjugated affinipure goat anti rabbit igg h l, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-occludin antibody Primary antibody against occludin Occludin

3 protocols using occludin antibody

Dextran Sulfate Sodium Colitis Model Protocol

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Dextran sodium sulfate
(DSS, molecular weight 36 000–50 000) was purchased
from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). l-Ergothioneine (EGT, ≥98%) was bought from Shanghai Aladdin
Biochemical Technology Co., Ltd. Fecal occult blood assay kit and
myeloperoxidase (MPO) assay kit were from Nanjing Jiancheng Bioengineering
Institute (Nanjing, China). Mouse IL-6, TNF-α ELISA kits, and
Goat anti-rabbit IgG were purchased from Boster Biological Technology
Co. Ltd. (Wuhan, China). The bicinchoninic acid (BCA) protein assay
kit was purchased from Solarbio Science & Technology Co., Ltd.
(Beijing, China). GADPH antibody was obtained from Abcam (Cambridge,
MA). The occludin antibody was purchased from Proteintech (Wuhan,
China). Fixable Viability Stain 780 from BD Pharmingen is useful for
discrimination of viable from nonviable cells. Antibodies for CD45,
CD3, CD4, CD11b, and CD16/CD32 (Fc Blocker) were bought from BD Pharmingen.
Antibody for F4/80 was from Biolegend. RPMI 1640 and fetal bovine
serum (FBS) were purchased from Gibco. Penicillin, streptomycin, and
glutamine were from Solarbio Life Technologies. Collagenase VIII and
DNAse I were from Sigma-Aldrich. Other chemicals were of analytical
grade.

Gao Y., Zhou B., Zhang H., Chen L., Wang X., Chen H, & Zhou L. (2022). l-Ergothioneine Exhibits Protective Effects against Dextran Sulfate Sodium-Induced Colitis in Mice. ACS Omega, 7(25), 21554-21565.

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Antioxidant and Anti-inflammatory Effects of Green Pea Hulls

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Green pea (ADM Dunn) hulls were provided by Canadian International Grains Institute (CIGI, Winnipeg, MB, Canada) on 21 June 2020. Chromatographic grade methanol, formic acid, and acetonitrile were purchased from Merck (Darmstadt, Germany). Quercetin standard and SCFAs (acetate, propionate, butyrate and valerate) were obtained from Aladdin (Shanghai, China). Dextran sulfate sodium (DSS, molecular weight 36–50 kDa) was purchased from MP Biomedicals (Irvine, CA, USA). The superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), malondialdehyde (MDA) and myeloperoxidase (MPO) assay kits were purchased from Nanjing Jiancheng Bioengineering Company (Nanjing, China). The enzyme-linked immunosorbent assay (ELISA) kits (TNF-a, IL-6, IL-1β and IL-10) were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). Claudin-1 antibody and Nrf2 antibody was purchased from Affinity (Changzhou, China). ZO-1 antibody and occludin antibody was purchased from Proteintech (Wuhan, China). Keap1 antibody were purchased from GeneTex (Irvine, CA, USA). Primers for glutamate-cysteine ligase catalytic subunit (GCLC), hemeoxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) were purchased from Tsingke (Beijing, China).

Guo F., Tsao R., Li C., Wang X., Zhang H., Jiang L., Sun Y, & Xiong H. (2021). Green Pea (Pisum sativum L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation. Foods, 10(11), 2765.

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Quantitative Analysis of Protein Expression

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MC-LR with a purity of 95% was purchased from Alexis Corporation (Lausen, Switzerland). RIPA buffer, bicinchoninic acid (BCA) protein assay kit was purchased from Beyotime (Shanghai, China). The polyvinylidene fluoride (PVDF) membrane was purchased from Merck Millipore Ltd., (Billerica, MA, USA). The phosphatase inhibitor cocktail and protease inhibitor cocktail were purchased from CWBIO (Beijing, China). Trizol Reagent was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The HiScript^® II Q RT SuperMix for qRT-PCR and ChamQ Universal SYBR qRT-PCR Master Mix were purchased from Vazyme (Nanjing, China). The ERK antibody, p-ERK antibody, Raf antibody, p-Raf antibody, ZO-1 antibody, Occludin antibody, Claudin1 antibody, β-actin antibody, HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L), and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) were purchased from Proteintech (Wuhan, China).

Yang Y., Zheng S., Chu H., Du C., Chen M., Emran M.Y., Chen J., Yang F, & Tian L. (2023). Subchronic Microcystin-LR Aggravates Colorectal Inflammatory Response and Barrier Disruption via Raf/ERK Signaling Pathway in Obese Mice. Toxins, 15(4), 262.

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