SEM analyses were conducted for the evaluation of dough samples at selected leavening times. Lyophilized dough slices [25 (link)] were placed on specimen holders and coated with gold by means of DC sputtering (Sputter and Carbon Coater Agar Scientific B7340). The microstructure of samples was observed at a magnification of 2000× with a LEO EVO 40 SEM (Zeiss, Oberkochen, Germany) with a 10 kV acceleration voltage. Representative micrographs from all the samples were selected. The microstructure of lyophilized wood oven pizza base samples was observed at a magnification of 2000× by a Phenom XL SEM (Thermo Scientific, Inc., Waltham, MA, USA) equipped with an ion sputter coater.
Leo evo 40 sem
Manufactured by Zeiss
Sourced in Germany, Brazil
The LEO EVO 40 SEM is a scanning electron microscope (SEM) designed for high-quality imaging and analysis of a wide range of materials. It features a thermionic tungsten electron gun, a high-resolution electron optical column, and advanced imaging capabilities. The LEO EVO 40 SEM is a versatile and reliable tool for researchers and technicians in various fields, including materials science, nanotechnology, and life sciences.
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Lab products found in correlation
2 protocols using leo evo 40 sem
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SEM Analysis of Dough and Pizza Microstructure
2
Scanning Electron Microscopy of Biological Samples
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Eggs fixed in Karnovsky's reagent was post-fixed with a solution of osmium tetroxide at 0.01 g/mL in a 0.05-M cacodylate buffer. Samples were then washed with distilled water and dehydrated with aqueous solutions of acetone in increasing concentrations (25, 50, 75, 90, and 100%) . Subsequently, samples were dried using the Critical Point Balzers CPD 030 apparatus. After drying, they were mounted on aluminum and gold stubs and brought to the SCD 050 Balzers evaporator to complete the preparation step. Samples were observed under a Leo Evo 40 SEM, using the Leo User Interface software to generate images (Carl Zeiss do Brasil, São Paulo, Brazil).
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