Lactosylceramides

Manufactured by AB Sciex

Sourced in United States

Lactosylceramides are a class of glycosphingolipids found in cell membranes. They function as structural components and are involved in cellular processes. The measurement of lactosylceramides can provide insights into various biological conditions.

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Lab products found in correlation

Debrisoquine, by Merck Group (2 mentions) Hexosylceramides, by AB Sciex (2 mentions) Fisher optima grade, by Thermo Fisher Scientific (2 mentions) High purity formic acid, by Thermo Fisher Scientific (2 mentions) 4 nitrobenzoic acid, by Merck Group (2 mentions) 18 1 chol d7 ester, by Avanti Polar Lipids (2 mentions) Equisplash lipidomix quantitative mass spec internal standard, by Avanti Polar Lipids (1 mentions)

2 protocols using lactosylceramides

Lipid Standards for LC-MS Analysis

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All LC-MS grade solvents, including acetonitrile and water, were purchased from Fisher Optima grade, Fisher Scientific. High-purity formic acid (99%) was purchased from Thermo-Scientific. Debrisoquine and 4-nitrobenzoic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). EquiSPLASH^® LIPIDOMIX^®, 15:0-18:1-d7-PA, C15 Ceramide-d7 (d18:1-d7/15:0) and 18:1 Chol (D7) ester were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Internal standards for free fatty acid (FFA), dihydroceramides (DCER), hexosylceramides (HCER), and lactosylceramides (LCER) were purchased from Sciex (Framingham, MA, USA) as Lipidyzer platform kit.

Bansal S., Li Y., Bansal S., Klotzbier W., Singh B., Jayatilake M., Sridharan V., Fernández J.A., Griffin J.H., Weiler H., Boerma M, & Cheema A.K. (2024). Genetic Upregulation of Activated Protein C Mitigates Delayed Effects of Acute Radiation Exposure in the Mouse Plasma. Metabolites, 14(5), 245.

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Comprehensive Lipidomics Analysis Protocol

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All LC-MS grade solvents including acetonitrile and water were purchased from Fisher Optima grade, Fisher Scientific. High purity formic acid (99%) was purchased from Thermo-Scientific. Debrisoquine and 4-nitrobenzoic acid were purchased from Sigma-Aldrich. EquiSPLASH^® LIPIDOMIX^® quantitative mass spec internal standard and 15:0-18:1-d7-PA, C15 Ceramide-d7 (d18:1-d7/15:0) and 18:1 Chol (D7) ester were purchased from Avanti polar lipids. Internal standard for free fatty acid (FFA), dihydroceramides (DCER), hexosylceramides (HCER), lactosylceramides (LCER) were purchased from Sciex as Lipidyzer platform kit. The LC-MS data acquisition details are provided in Supplementary data.

Bansal S., Bansal S., Fish B.L., Li Y., Xu X., Fernandez J.A., Griffin J.H., Himburg H.A., Boerma M., Medhora M, & Cheema A.K. (2023). Analysis of the Urinary Metabolic Profiles in Irradiated Rats treated with Activated Protein C (APC), a Potential Mitigator of Radiation Toxicity. International journal of radiation biology, 99(7), 1109-1118.

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