Protein extraction and western blot were performed according to published methods.18 The following primary antibodies were used: rabbit anti‐human PIG3 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti‐human p‐FAK (Tyr397) monoclonal antibody, rabbit anti‐human p‐FAK (Tyr576) polyclonal antibody, rabbit anti‐human p‐FAK (Tyr925) polyclonal antibody, rabbit anti‐human total FAK monoclonal antibody, rabbit anti‐human p‐Src (Tyr416) monoclonal antibody, rabbit anti‐human total Src monoclonal antibody (Cell Signaling Technology, Beverly, MA, USA) and mouse anti‐human GAPDH monoclonal antibody (Beyotime Institute of Biotechnology, Haimen, China) as the loading control. All primary antibodies were used at a 1000‐fold dilution.
Rabbit anti human pig3 polyclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Rabbit anti-human PIG3 polyclonal antibody is a laboratory reagent used for the detection and analysis of the PIG3 protein in human samples. This antibody is produced in rabbits and recognizes the PIG3 protein, which is involved in the cellular response to DNA damage. The antibody can be used in various immunoassays, such as Western blotting and immunohistochemistry, to study the expression and localization of the PIG3 protein.
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Lab products found in correlation
2 protocols using rabbit anti human pig3 polyclonal antibody
1
Western Blot Analysis of Protein Signaling
2
Immunohistochemical Localization of PIG3
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PIG3 localization was evaluated by immunohistochemistry (IHC) as previously described.18 Rabbit anti‐human PIG3 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used at a 100‐fold dilution.
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