Ckx rcd microscope

To study the effect of A‐PRF‐XBSM mixture exudates on cell migration, a scratch wound‐healing assay mimics cell migration during wound healing in vitro. hPDLSCs were seeded into six‐well plates (3 × 10⁵ cells/well; Nunc, Denmark) and cultured until reaching 80% confluence. A scratch was introduced into the single‐layer on each dish using a pipette tip (100–1000 µl tip; Thermo Scientific, USA). Non‐adherent cells were extracted by washing once with 1× phosphate‐buffered saline (PBS) (Gibco, USA). The cells were then serum‐starved overnight. The A‐PRF‐XBSM mixture exudates were added to each well. Complete medium and DMEM/F12 without FBS were used as positive and negative controls, respectively. The images of cell migration into the scratch area at 0 and 24 h were taken using an Olympus CKX‐RCD microscope (Olympus, Japan) set up with a DP2‐BSW microscope camera and then analyzed using ImageJ 1.52a software (USA).

The BPS and HepBPS were fixed with 10% formalin, embedded in paraffin, and processed for staining with hematoxylin and eosin (H&E). Images were taken using Olympus CKX-RCD microscope (Tokyo, Japan) equipped with a DP2-BSW microscope digital camera.