lncMGPF promoter regions were cloned into a PGL3‐basic luciferase reporter vector (Addgene, USA), and the constructed luciferase reporter vectors were transfected into C2C12 myoblasts in a 24‐well plate. The 3′ untranslated region (UTR) of MEF2C, sequences containing the miR‐135a‐5p binding site of lncMGPF or AK394747, and the sequences of lncMGPF or AK394747 with mutated miR‐135a‐5p binding sites, were cloned into the pmirGLO Dual‐Luciferase miRNA Target Expression Vector (Promega, USA) to construct the corresponding reporter vectors. Those reporter vectors were transfected into C2C12 myoblasts, HeLa cells, PK cells, and myogenic progenitor cells using Lipofectamine 2000. The luciferase assay was performed using the Dual‐Luciferase Reporter Assay System (Promega, USA) and the enzymatic activity of luciferase measured using a PerkinElmer 2030 Multilabel Reader (PerkinElmer, USA).
Pgl3 basic luciferase reporter vector
Manufactured by Addgene
Sourced in United States
The PGL3-basic luciferase reporter vector is a plasmid DNA construct designed for the expression and measurement of luciferase activity in eukaryotic cells. It contains a multiple cloning site upstream of the luciferase gene, allowing for the insertion of regulatory sequences to study their effect on gene expression.
Automatically generated - may contain errors
2 protocols using pgl3 basic luciferase reporter vector
1
Luciferase Assay for lncRNA Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
lncMGPF promoter regions were cloned into a PGL3‐basic luciferase reporter vector (Addgene, USA), and the constructed luciferase reporter vectors were transfected into C2C12 myoblasts in a 24‐well plate. The 3′ untranslated region (UTR) of MEF2C, sequences containing the miR‐135a‐5p binding site of lncMGPF or AK394747, and the sequences of lncMGPF or AK394747 with mutated miR‐135a‐5p binding sites, were cloned into the pmirGLO Dual‐Luciferase miRNA Target Expression Vector (Promega, USA) to construct the corresponding reporter vectors. Those reporter vectors were transfected into C2C12 myoblasts, HeLa cells, PK cells, and myogenic progenitor cells using Lipofectamine 2000. The luciferase assay was performed using the Dual‐Luciferase Reporter Assay System (Promega, USA) and the enzymatic activity of luciferase measured using a PerkinElmer 2030 Multilabel Reader (PerkinElmer, USA).
+ Expand
lncMGPF promoter regions were cloned into a PGL3‐basic luciferase reporter vector (Addgene, USA), and the constructed luciferase reporter vectors were transfected into C2C12 myoblasts in a 24‐well plate. The 3′ untranslated region (UTR) of MEF2C, sequences containing the miR‐135a‐5p binding site of lncMGPF or AK394747, and the sequences of lncMGPF or AK394747 with mutated miR‐135a‐5p binding sites, were cloned into the pmirGLO Dual‐Luciferase miRNA Target Expression Vector (Promega, USA) to construct the corresponding reporter vectors. Those reporter vectors were transfected into C2C12 myoblasts, HeLa cells, PK cells, and myogenic progenitor cells using Lipofectamine 2000. The luciferase assay was performed using the Dual‐Luciferase Reporter Assay System (Promega, USA) and the enzymatic activity of luciferase measured using a PerkinElmer 2030 Multilabel Reader (PerkinElmer, USA).
2
Promoter-Driven Luciferase Reporting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Different fragments of the LncEDCH1 promoter were cloned into a pGL3-basic luciferase reporter vector (Addgene, USA), and the constructed luciferase reporter vectors were transfected into CPMs in a 96-well plate. The luciferase assay was performed using the Dual-Luciferase Reporter Assay System (Promega, USA) following the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!