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Lead nitrate

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Lead nitrate is an inorganic compound with the chemical formula Pb(NO3)2. It is a white, crystalline solid that is commonly used in various laboratory applications.

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Lab products found in correlation

Sodium succinate, by Merck Group (2 mentions) Decylubiquinol, by Merck Group (2 mentions) Metformin hydrochloride, by Merck Group (2 mentions) Coomassie brilliant blue g 250, by Bio-Rad (2 mentions) Tricine, by Avantor (2 mentions) Immunoblotting membrane, by Bio-Rad (2 mentions) Ecl western blot detection kit, by Bio-Rad (2 mentions) N dodecyl β d maltoside, by Merck Group (2 mentions) Amino n caproic acid, by Avantor (2 mentions) Serva blue g 250, by Serva Electrophoresis (2 mentions) Sds page protein standard markers, by Bio-Rad (2 mentions) Nitro blue tetrazolium tablets, by Merck Group (2 mentions) Tris base, by Avantor (2 mentions) Streptozotocin (stz), by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Cytochrome c, by Merck Group (2 mentions) Glucose 6 phosphate (g6p), by Merck Group (2 mentions) Sodium cyanide, by Merck Group (2 mentions)

3 protocols using lead nitrate

1

Platelet Activation Signaling Pathway Analysis

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Sepharose CL‐2B column, bovine thrombin, and Ca2+‐ionophore calcimycin were from Sigma‐Aldrich. CVX was the product of Pentapharm. Phycoerythrin (PE)‐labeled anti‐CD62 antibody (Ab), PE‐cyanine5 (PECy5)‐conjugated anti‐CD41a Ab, PE‐labeled mouse IgG1, fluorescein isothiocyanate (FITC)‐labeled mouse IgG2a, PE‐conjugated annexin V, and annexin V binding buffer were purchased from Becton Dickinson. Mouse anti‐human CD41a Ab, Alexa‐fluor 568 annexin V conjugate, and DyLight 405‐labeled goat anti‐mouse Ab were the products of Thermo Fisher Scientific. DyLight 488‐labeled horse anti‐rabbit Ab, Vectashield® antifade mounting medium, and normal goat serum were from Vector Laboratories. Goat anti‐rabbit IgG conjugated to 15 nm gold particles and goat anti‐mouse IgG conjugated to 10 nm gold particles were purchased from BBI Solutions. Uranyl acetate was from Electron Microscopy Sciences. Lead citrate was made from lead nitrate (VWR International Ltd.). Rabbit anti‐human FXIII‐A Ab and FITC‐labeled mouse anti‐human‐FXIII‐A Ab were produced in our laboratories.16, 22 The fluorescent calcium indicator Fluo‐4‐AM was obtained from Thermo Fisher Scientific. The RhoA inhibitor Rhosin hydrochloride was purchased from Tocris Bioscience and the transglutaminase inhibitor T101 from Zedira.
Somodi L., Beke Debreceni I., Kis G., Cozzolino M., Kappelmayer J., Antal M., Panyi G., Bárdos H., Mutch N.J, & Muszbek L. (2022). Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles. Journal of Thrombosis and Haemostasis, 20(5), 1223-1235.
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2

Mitochondrial Enzyme Activity Assay

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Streptozotocin (STZ), NADH, NAD+, ATP, cytochrome c, antimycin A, sodium cyanide, decylubiquinol, n-dodecyl-beta-D-maltoside, sodium succinate, nitro blue tetrazolium (NBT) tablets, EDTA, dimethyl sulfoxide, dichlorophenolindophenol (DCPIP), NADPH, glucose-6-phosphate, and metformin hydrochloride were purchased form Sigma (St. Louis, MO). Lead nitrate, tricine, amino- N- caproic acid, Tris base, and Bis-tris were obtained from VWR International. Serva blue G-250 was purchased from Serva (Heidelberg, Germany). Coomassie brilliant blue G-250, SDS-PAGE protein standard markers, ECL Western blot detection kit, and immunoblotting membrane were purchased from Bio-Rad (Richmond, CA).
Wu J., Luo X., Thangthaeng N., Sumien N., Chen Z., Rutledge M.A., Jing S., Forster M.J, & Yan L.J. (2017). Pancreatic mitochondrial complex I exhibits aberrant hyperactivity in diabetes. Biochemistry and Biophysics Reports, 11, 119-129.
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3

Mitochondrial Enzyme Activity Assay

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Streptozotocin (STZ), NADH, NAD+, ATP, cytochrome c,
antimycin A, sodium cyanide, decylubiquinol, n-dodecyl-beta-D-maltoside, sodium
succinate, nitro blue tetrazolium (NBT) tablets, EDTA, dimethyl sulfoxide,
dichlorophenolindophenol (DCPIP), NADPH, glucose-6-phosphate, and metformin
hydrochloride were purchased form Sigma (St. Louis, MO). Lead nitrate, tricine,
amino- N- caproic acid, Tris base, and Bis-tris were obtained from VWR
International. Serva blue G-250 was purchased from Serva (Heidelberg, Germany).
Coomassie brilliant blue G-250, SDS-PAGE protein standard markers, ECL Western
blot detection kit, and immunoblotting membrane were purchased from Bio-Rad
(Richmond, CA).
Wu J., Luo X., Thangthaeng N., Sumien N., Chen Z., Rutledge M.A., Jing S., Forster M.J, & Yan L.J. (2017). Pancreatic mitochondrial complex I exhibits aberrant hyperactivity in diabetes. Biochemistry and Biophysics Reports, 11, 119-129.
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