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Penicillin streptomycin amphotericin b psf

Manufactured by Lonza
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Penicillin-streptomycin-amphotericin B (PSF) is a laboratory reagent used to supplement cell culture media. It contains a combination of the antibiotics penicillin, streptomycin, and the antifungal agent amphotericin B. The function of this product is to provide broad-spectrum antimicrobial protection for cell cultures, preventing bacterial and fungal contamination.

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Lab products found in correlation

Human recombinant epidermal growth factor, by Thermo Fisher Scientific (2 mentions) Epilife, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (2 mentions) Epilife defined growth supplement edgs, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

3 protocols using penicillin streptomycin amphotericin b psf

1

Isolation and Culture of Primary Respiratory Epithelial Cells

Cited 2 times
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Primary RCFs were isolated as previously described and used between passages 3 and 7 [26 (link),27 (link)]. RCFs were cultured in Dulbecco’s modified medium (DMEM) low glucose with 10% fetal bovine serum (Atlanta Biologicals, Lawrence, GA, USA) and 1% penicillin-streptomycin-amphotericin B (PSF; Lonza, Walkersville, MD, USA).
Rozo V., Quan M., Aung T., Kang J., Thomasy S.M, & Leonard B.C. (2022). Andrographolide Inhibits Corneal Fibroblast to Myofibroblast Differentiation In Vitro. Biomolecules, 12(10), 1447.
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2

Immortalized Corneal Epithelial Cell Culture

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Human telomerase reverse transcriptase-immortalized corneal epithelial (hTCEpi) cells graciously donated by James Jester, PhD (University of California Irvine), were used between passage 36 and 50. The hTCEpi cells were cultured in growth medium composed of EpiLife® (Life Technologies; Carlsbad, CA) supplemented with 1% EpiLife Defined Growth Supplement (EDGS®; a proprietary combination of bovine serum albumin, bovine transferrin, hydrocortisone, recombinant human-like growth factor type-1, prostaglandin, and recombinant human epidermal growth factor (Life Technologies) and 1% penicillin-streptomycin-amphotericin B (PSF, Lonza; Walkersville, MD).
Domanico M., Fukuto A., Tran L.M., Bustamante J.M., Edwards P.C., Pinkerton K.E., Thomasy S.M, & Van Winkle L.S. (2022). Cytotoxicity of 2D engineered nanomaterials in pulmonary and corneal epithelium. NanoImpact, 26, 100404.
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3

Immortalized Corneal Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human telomerase reverse transcriptase-immortalized corneal epithelial (hTCEpi) cells graciously donated by James Jester, PhD (University of California Irvine), were used between passage 36 and 50. The hTCEpi cells were cultured in growth medium composed of EpiLife® (Life Technologies; Carlsbad, CA) supplemented with 1% EpiLife Defined Growth Supplement (EDGS®; a proprietary combination of bovine serum albumin, bovine transferrin, hydrocortisone, recombinant human-like growth factor type-1, prostaglandin, and recombinant human epidermal growth factor (Life Technologies) and 1% penicillin-streptomycin-amphotericin B (PSF, Lonza; Walkersville, MD).
Domanico M., Fukuto A., Tran L.M., Bustamante J.M., Edwards P.C., Pinkerton K.E., Thomasy S.M, & Van Winkle L.S. (2022). Cytotoxicity of 2D engineered nanomaterials in pulmonary and corneal epithelium. NanoImpact, 26, 100404.
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