Splenocytes were cultured in RPMI 1640 medium (from Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum, 50 units/mL penicillin, and 50 µg/mL streptomycin (from Fisher Scientific, Waltham, MA). Lipopolysaccharide (LPS) was obtained from Sigma-Aldrich (Burlington, MA). The following antibodies were obtained from Biolegend (San Diego, CA) and used for flow cytometry analysis: FITC anti-mouse CD11b (clone: 29F.1A12), PE-CF594 anti-mouse F4/80 (clone:BM8), PerCPCy5.5 anti-mouse CD11c (clone: N418), PE anti-mouse CD86 (clone: GL-1), BV421 anti- mouse MHC-class II (clone: M5/114.15.2). The following antibodies were obtained from Biolegend (San Diego, CA) and used for flow cytometry analysis: FITC anti-mouse CD11b (clone: 29F.1A12), PE-CF594 anti-mouse F4/80 (clone: T45-2342), BV605 anti-mouse Ly6C (clone: HK1.4), BV711 anti-mouse Ly6G (clone: 1A8) PerCPCy5.5 anti-mouse CD11c (clone: N418), PE anti-mouse CD86 (clone: GL-1), BV421 anti- mouse MHC-class II (clone: M5/114.15.2).
Torres-Sanchez A., Rivera-Robles M., Castillo-Pichardo L., Martínez-Ferrer M., Dorta-Estremera S.M, & Dharmawardhane S. (2023). Rac and Cdc42 inhibitors reduce macrophage function in breast cancer preclinical models. Frontiers in Oncology, 13, 1152458.
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