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Monoclonal mouse anti flag

Manufactured by Abcam
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Monoclonal mouse anti-Flag is a laboratory reagent used for the detection and purification of proteins tagged with the FLAG epitope. It is a mouse-derived monoclonal antibody that recognizes the FLAG peptide sequence (DYKDDDDK).

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Lab products found in correlation

Polyclonal rabbit anti hif 1α, by Abcam (2 mentions) Polyclonal rabbit anti vhl, by Proteintech (2 mentions) Mouse monoclonal anti ha, by Cell Signaling Technology (2 mentions) Chx a8244, by Apexbio (2 mentions) Bay85 3934, by Apexbio (2 mentions) Mouse monoclonal anti gapdh, by Abcam (2 mentions) Rabbit polyclonal anti caspase 3, by Cell Signaling Technology (2 mentions) Mg132 a2585, by Apexbio (2 mentions) Polyclonal goat anti septin4, by Abcam (2 mentions)

2 protocols using monoclonal mouse anti flag

1

Protein Expression and Analysis

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Polyclonal goat anti-Septin4 (abcam, USA), polyclonal rabbit anti-HIF-1α (abcam), polyclonal rabbit anti-VHL (proteintech, USA), polyclonal rabbit anti-caspase-3 (Cell Signaling Technology, USA), monoclonal mouse anti-HA (Cell Signaling Technology), monoclonal mouse anti-Flag (abcam), monoclonal rabbit anti-β-tubulin (Proteintech), and monoclonal mouse anti-GAPDH (abcam). CHX (A8244) and MG132 (A2585), used in DMSO, were purchased from ApexBio (USA). HIF-PHDs inhibitor BAY85-3934 was also purchased from ApexBio.
Wu S., Zhang Y., You S., Lu S., Zhang N, & Sun Y. (2021). Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α. Cell Death Discovery, 7, 172.
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2

Dissecting Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal goat anti-Septin4 (abcam, USA), polyclonal rabbit anti-HIF-1α (abcam), polyclonal rabbit anti-VHL (proteintech, USA), polyclonal rabbit anti-caspase-3 (Cell Signaling Technology, USA), monoclonal mouse anti-HA (Cell Signaling Technology), monoclonal mouse anti-Flag (abcam), and monoclonal mouse anti-GAPDH (abcam). CHX (A8244) and MG132 (A2585), used in DMSO, were purchased from ApexBio (USA). HIF-PHDs inhibitor BAY85-3934 was also purchased from ApexBio.
Wu S., Zhang Y., You S., Lu S., Zhang N., & Sun Y. (2020). Septin4 Aggravates Hypoxia-Induced Cardiomyocytes Injury by Promoting HIF-1α Ubiquitination and Degradation through VHL.
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