λ bacteriophage genomic dna rapid extracted kit

To increase our understanding of the phage at the genomic level, the phage DNA was extracted as previously described (Chen et al., 2019 (link)). First, phage particles were treated with DNase I and RNase A (New England BioLab, England) followed by incubating at 37°C for 30 min to remove any exogenous host genomic DNA and RNA. Then phage PVA8 genomic DNA was extracted using the λ Bacteriophage Genomic DNA Rapid Extracted Kit (Shanghai Yuanye Biotechnology Co., Ltd., China) following the manufacturer^’s instructions. The purified phage PVA8 DNA was performed on an Illumina NovaSeq sequencing platform (Qingdao Weilai Biotechnology Co., Ltd., China). The filtered reads were assembled using SOAPdenovo by the default parameters. The complete genome of phage PVA8 was finished and then manually inspected.

First, the concentrated phage PVA23 particles were treated with DNase I and RNase A (New England BioLab, England) followed by incubating at 37 °C for 30 min to remove any exogenous host genomic DNA and RNA. Then, phage PVA23 genomic DNA was extracted using the λ Bacteriophage Genomic DNA Rapid Extracted Kit (Yuanye Biotechnology Co., Ltd. of Shanghai, Shanghai, China) following the manufacturer’s instructions. The purified phage PVA23 DNA was performed on an Illumina NovaSeq sequencing platform (Weilai Biotechnology Co., Ltd. of Qingdao, Qingdao, China). The filtered reads were assembled using SOAPdenovo by the default parameters. The complete genome of phage PVA23 was finished and then manually inspected.