Wild-type HAP1 C631 cells were ordered from Horizon Discovery and maintained at 37 °C, 5% CO2 in IMDM (Life Technologies) supplemented with 10% FCS. For stable Cas9 expression, the parental cell line was transduced at an MOI of ~ 0.5 with lentivirus generated using the Lenti Cas9-2A-Blast plasmid (kind gift of the Moffat lab, Addgene #73310) and selected with 20 μg/ml blasticidin (InvivoGen) for at least 1 week. Out of this bulk population, single cells with a small cell size (indicative for a haploid genotype) were sorted in 96-well plates, expanded, and again selected with 20 μg/ml blasticidin. blasticidin-resistant clones were further characterized for Cas9 editing efficiency. Mycoplasma contamination was periodically assessed for all cell lines. The HAP1 cell line was authenticated using Multiplex Cell Authentication by Multiplexion (Heidelberg, Germany) as described recently [68 (link)]. The SNP profile was unique.
Hap1 c631 cells
Manufactured by Horizon Discovery
HAP1 C631 cells are a population of haploid human cell lines derived from the chronic myelogenous leukemia (CML) cell line KBM-7. These cells contain a single copy of each chromosome and are commonly used for genetic studies and loss-of-function screening.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hap1 c631 cells
1
Generating Stable Cas9-Expressing HAP1 Cells
2
Establishing Stable Cas9 Expression in HAP1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Wild-type HAP1 C631 cells were ordered from Horizon Discovery and maintained at 37˚C, 5%
CO 2 in IMDM (Life Technologies) supplemented with 10% FCS. For stable Cas9 expression, the parental cell line was transduced at an MOI of ~0.5 with lentivirus generated using the Lenti Cas9-2A-Blast plasmid (kind gift of the Moffat lab, Addgene #73310) and selected with 20 µg/ml blasticidin (InvivoGen) for at least one week. Out of this bulk population, single cells with a small cell size (indicative for a haploid genotype) were sorted in 96-well plates, expanded and again selected with 20 µg/ml blasticidin. blasticidin-resistant clones were further characterized for Cas9 editing efficiency. Mycoplasma contamination was periodically assessed for all cell lines. The HAP1 cell line was authenticated using Multiplex Cell Authentication by Multiplexion (Heidelberg, Germany) as described recently (Castro et al. 2012) . The SNP profile was unique.
CO 2 in IMDM (Life Technologies) supplemented with 10% FCS. For stable Cas9 expression, the parental cell line was transduced at an MOI of ~0.5 with lentivirus generated using the Lenti Cas9-2A-Blast plasmid (kind gift of the Moffat lab, Addgene #73310) and selected with 20 µg/ml blasticidin (InvivoGen) for at least one week. Out of this bulk population, single cells with a small cell size (indicative for a haploid genotype) were sorted in 96-well plates, expanded and again selected with 20 µg/ml blasticidin. blasticidin-resistant clones were further characterized for Cas9 editing efficiency. Mycoplasma contamination was periodically assessed for all cell lines. The HAP1 cell line was authenticated using Multiplex Cell Authentication by Multiplexion (Heidelberg, Germany) as described recently (Castro et al. 2012) . The SNP profile was unique.
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