All flow cytometry analysis was conducted in the University of Minnesota Flow Cytometry Core Facility using BD LSR II and Fortessa cytometers (BD, San Jose, CA). For surface staining, cells were stained for 20 minutes with 1:100 dilutions of fluorochrome-conjugated antibodies prior to washing and analysis or intracellular staining. Anti-mouse CD4 (GK1.5), CD8α (53-6.7), CD25 (PC61.5), FOXP3 (FJK-16s), Thy1.2 (30-H12 or 53.21), CD73 (TY/11.8), CD45.2 (104), CD45.1 (A20), Thy1.1 (HIS51), CD44 (IM7), IL2 (JES6-5H4), CD11c (N418), CD19 (1D3), NK1.1 (PK136), EpCAM (G8.8), CD172α (P84) and CD62L (MEL14) were from eBioscience (San Diego, CA). CD4 (GK1.5, BV786), CD45RA (14.8) and CD172α (P84) were from BD Biosciences (San Jose, CA). CD3 (17A2 or 145-2C11) and Rat IgG1 (HPRN) were from Tonbo Biosciences (San Diego, CA). UEA1 was from Vector Laboratories (Burlingham, CA). Intracellular detection of FOXP3 was performed as previously described (14 (link)) using the eBioscience Transcription Factor staining kit. Intracellular detection of IL2 was performed with BD Cytofix/Cytoperm kit as described by the manufacturer.
Cd172α p84
Manufactured by BD
CD172α (P84) is a cell surface glycoprotein expressed on myeloid cells, including monocytes, macrophages, and dendritic cells. It functions as an inhibitory receptor that regulates the activation of these immune cells.
Automatically generated - may contain errors
Lab products found in correlation
Fortessa, by BD (1 mentions)
Lsr 2, by BD (1 mentions)
Uea 1, by Vector Laboratories (1 mentions)
Cd62l mel 14, by Thermo Fisher Scientific (1 mentions)
Transcription factor staining kit, by Thermo Fisher Scientific (1 mentions)
Cytofix cytoperm kit, by BD (1 mentions)
Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions)
Cd19 ebio1d3, by Thermo Fisher Scientific (1 mentions)
2 protocols using cd172α p84
1
Flow Cytometry Immunophenotyping Protocol
2
Bone Marrow Multiparameter Analysis
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Bone marrow was flushed from the femur and tibia of one leg and was used after red blood cell lysis using eBioscience RBC lysis buffer. Multi-parameter analyses of labeled cell suspensions were performed on an LSR II (Becton Dickinson) and data were analyzed with FlowJo software (TreeStar). Fluorochrome- or biotin-conjugated monoclonal antibodies (mAbs) to the following were used: mouse IA/IE (M5/114.15.2) and CD172α (P84) (both from BD Biosciences); CD11c (N418), CD209a (MMD3), CD117 (2B8), Ly6C (HK1.4), SiglecH (440c), B220 (RA3-6B2), CD135 (A2F10), CD49b (DX5) and CD19 (eBio1D3) (all from eBioscience); Ly6G (1A8) and CD3e (145-2C11); CD74 (OX6) (from Abcam); and CX3CR1 (Catalog # FAB5825P) (from RnD Systems). The streptavidin–phycoerythrin-CF594 conjugate (25-4317-82) was from BD Biosciences. The dilution of antibodies was as follows: CD117 (2B8) 1:100, CD135 (A2F10) 1:100, CD74 (OX6) 1:50, CX3CR1 (Catalog # FAB5825P) 1:50, streptavidin–phycoerythrin-CF594 conjugate (25-4317-82) 1:400, all other antibodies 1:200.
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