Lentil lectin, wheat germ agglutinin (WGA), maackia amurensis lectin (MAL), peanut lectin, sambucus sieboldiana lectin (SSL) were purchased from Wako (Japan). The lectin from Datura stramonium (DSL, jimson weed, thorn apple), succinyl-concanavalin A (succ-Con A), lectin from Galanthus nivalis (snowdrop, GNL), erythroagglutinin PHA-E, leucoagglutinin PHA-L, phytohaemagglutinin PHA-M and phytohaemagglutinin PHA-P from Phaseolus vulgaris (red kidney bean) were purchased from Sigma Aldrich. The carbohydrate specificity of lectins are as follows: lentil lectin and succ-Con A specifically bind to the Man/GlcNAc/Glc [20 (link)], WGA specifically binds to the GlcNAc/Neu5Ac [20 (link)], MAL binds to the Neu5Acα3Gal [21 (link)], SSL binds to the Neu5Acα6Gal/GalNAc [20 (link), 21 (link)], DSL binds to the Galβ3GlcNAc [22 (link)], GNL binds to manα3man [23 (link)], peanut lectin binds to Gal/GalNAc [22 (link)], PHA-E, PHA-L, PHA-M and PHA-L bind to complex-type N-glycans [24 (link)].
The SARS-COV-2 S trimer expressed in HEK293 cells were purchased from ACROBiosystems (Beijing, China).
Wang W., Li Q., Wu J., Hu Y., Wu G., Yu C., Xu K., Liu X., Wang Q., Huang W., Wang L, & Wang Y. (2021). Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants. Emerging Microbes & Infections, 10(1), 1519-1529.
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