The largest database of trusted experimental protocols

13 protocols using acetic acid

1

Populus tomentosa Wood Modification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Populus tomentosa plank (Zibo, China, with air-dry density 450–500 kg/m3) was cut into blocks (L × T × R) of 30 mm × 30 mm × 5 mm. In addition, 3-aminopropyl triethoxysilane (APTES, 98%) and Methyltriethoxysilane (MTES, 98%) were provided by Aladdin Biochemical Technology in Shanghai, China. Copper chloride dihydrate (AR), acetic acid (AR), and ethanol were purchased from Guangzhou Chemical Reagent Factory and used without further purification.
+ Open protocol
+ Expand
2

Phytochemical Profiling and Antioxidant Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetone, n-hexane, ethyl acetate, concentrated sulfuric acid, sodium hydroxide, phosphoric acid, acetonitrile, ascorbic acid, methanol, formic acid, sodium acetate, acetic acid, hydrochloric acid, iron(III) chloride hexahydrate solution, ammonium acetate, were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). phenolic compound standards [rutin (Rut), quercetin (Que), quercitrin (Quer), isoquercitrin (Iso), chlorogenic acid (ChA), syringic acid (SyA), ferulic acid (FeA), caffeic acid (CaA), resveratrol (Res), epicatechin (Epi), astragalin (Ast), scopoletin (Sco), galangal (Gal), catechuic acid (CatA), vanillic acid (VaA), benzoic acid (BeA), gallic acid (GaA), and protocatechuic acid (PrA)] were purchased from the National Institutes for Food and Drug Control (Beijing, China). 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2ʹ-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
+ Open protocol
+ Expand
3

Synthesis of Metal-Organic Frameworks

Check if the same lab product or an alternative is used in the 5 most similar protocols
FeCl3·6H2O was obtained from McLean Biochemical
Technology Co., Ltd., Shanghai. NH2-BDC, Congo red, and
NaOH were purchased from Aladdin. Acetic acid, ethanol, N,N-dimethylformamide (DMF), and AgNO3 were bought from Guangzhou Chemical Reagent Factory. Sodium anhydrous
acetate was obtained from Guangdong Guanghua Chemical Factory Co,
Ltd. NaBH4 was bought from Tianjin Damao Chemical Reagent
Factory.
+ Open protocol
+ Expand
4

Quantification of Bacterial Signaling Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The standards N-butyryl-L-HSL (C4-HSL) and N-(3-oxododecanoyl)-L-HSL (3-oxo-C12-HSL) were purchased from Sigma. Methanol, acetonitrile (HPLC grade), and formic acid (MS grade) were purchased from Fisher Scientific (Loughborough, UK). Acetic acid and ethyl acetate (HPLC grade) were obtained from Guangzhou Chemical Reagent Factory (Guangzhou, China), and ultra-pure water (>18 MΩ/cm) was obtained from a Milli-Q water Elga Maxima water purification system (Merck KgaA, Germany).
+ Open protocol
+ Expand
5

Investigating Bevacizumab's Cellular Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bevacizumab (Roche Diagnostics GmbH., Mannheim, Germany, Av), acetic
acid, nitric acid, perchloric acid, sodium chloride, potassium chloride and
magnesium chloride were purchased from Guangzhou Chemical Reagent Factory.
Thiazolyl blue tetrazolium bromide (MTT), DMSO, phosphate buffer, DMEM media,
ascorbic acid, Na2SeO3, ethyleediaminetetraacetic acid
(EDTA) and 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) were procured
from Sigma-Aldrich. EDTA-free mini protease inhibitor tablet was purchased from
Thermo Scientific. The ultrapure water from Milli-Q water purification system
(Milipore) was used in all experiments.
+ Open protocol
+ Expand
6

Multi-analyte quantification protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
TYL was brought from Hengtong Guanghua Factory (Xi’an, China), and ROX, SPM, JOS, and CLA were from European Pharmacopoeia (EDQM, Strasbourg, France). KIT, ERY, TIL, TUL, and AZI were obtained from Sigma Chemicals Co. (St. Louis, MO, USA). MED was obtained from the China Institute of veterinary drugs control (Beijing, China). The purity of each analytical standard is higher than 92.5%. The chromatographic grade methanol, acetonitrile, and formic acid (FA) were purchased from Fisher Scientific (Fairlawn, NJ, USA). Other organic solvents such as ammonium hydroxide, acetic acid, and chloroform were analytical grade and brought from Guangzhou Chemical Reagent Factory (Guangzhou, China). Ultrapure deionized water was produced by a Millipore MilliQ water system (Molsheim, France). C18 (200 mg, 3 mL) and SCX (60 mg, 3 mL) SPE cartridges were available from Agilent Technologies Co. (Santa Clara, CA, USA). The Oasis HLB cartridge (60 mg, 3 mL) was obtained from Waters Co. (Milford, MA, USA).
The stock standard solution (1.0 mg/mL) of each compound was prepared by dissolving 10 mg of analytical standard in 10 mL of methanol and can be stored at −20 °C for 3 months. Working solutions were obtained by diluting the stock solution with methanol, daily, according to practical requirements.
+ Open protocol
+ Expand
7

Detailed Reagent Procurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vanillin was purchased Shanghai Sangon Biological Engineering Technology (Shanghai, China). o-Toluidine and N, N-dimethylformamide were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Sodium citrate tribasic dihydrate and Tris were purchased from Sigma-Aldrich (Steinheim, Germany). Glycine was purchased from GenStar Biosolutions Co., Ltd. (Beijing, China). Citric acid, disodium hydrogen, HCl, NaOH, Na2HPO4 and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). Acetonitrile was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). n-Hexane was purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Other chemicals were purchased from standard commercial sources and were of analytical grade purity.
+ Open protocol
+ Expand
8

Chitosan Deacetylation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
CS (deacetylation: 80%–85%, ~100 kDa) was purchased from Yuhuan Ocean Biochemical Co. (Zhejiang, People’s Republic of China). TPP, zinc sulfate, H2O2, and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, People’s Republic of China). Sodium hydroxide was purchased from Guangdong Guanghua Chemical Reagent Factory (Shantou, People’s Republic of China). Calcium chloride was purchased from Tianjin Guangcheng Chemical Co. Ltd. (Tianjin, People’s Republic of China). Copper sulfate, Tween 80, and ethanol were purchased from Tianjin Yongda Chemical Co. Ltd. (Tianjin, People’s Republic of China). Ferric sulfate was purchased from Tianjin Fuchen Chemical Co. Ltd. (Tianjin, People’s Republic of China). Distilled water was supplied by Guangzhou Watson’s Food & Drinks Co. Ltd. (Guangzhou, People’s Republic of China). Nile red was purchased from Guangzhou Qiyun Biotechnology Co. Ltd. (Guangzhou, People’s Republic of China). All the other reagents were of analytical grade.
+ Open protocol
+ Expand
9

Western Blot Analysis of ERβ Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Taq Master Mix (SinoBio, Shanghai, China), agarose (Biowest, Nuaille, France), sterile double distilled water, sterile double distilled water, monoclonal rabbit β-actin antibody (dilution, 1:5,000; cat. no. MA5-15739; Invitrogen Life Technologies, Carlsbad, CA, USA), polyclonal rabbit ERβ antibody (dilution, 1:500; cat. no. BD-PT1637; BioLegend, Inc., San Diego, CA, USA), monoclonal rabbit phosphorylation ERβ antibody (dilution, 1:1,000; cat. no. orb10614; Cell Signaling Technology, Inc., Danvers, MA, USA), 0.9% stroke-physiological saline solution (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), pentobarbital sodium (Guangzhou Chemical Reagent Factory, Guangzhou, China), TRIzol (Invitrogen Life Technologies), AngII (RayBiotech, Norcross, GA, USA), and acetic acid (Guangzhou Chemical Reagent Factory) constituted the reagents used in this study. Phosphate buffer was purchased from Gibco Life Technologies (Carlsbad, CA, USA).
+ Open protocol
+ Expand
10

Simultaneous Determination of Eight Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Metformin Hydrochloride (C4H11N5·HCl, purity 100.0%), glyburide (C23H28ClN3O5S, purity > 99.9%), tolbutamide (C12H18N2O3S, purity 98%), enalapril maleate (C20H28N2O5·C4H4O4, purity 99.8%), indapamide (C16H16ClN3O3S, purity 98.2%), nifedipine (C17H18N2O6, purity 99.8%), atorvastatin calcium (C66H68CaF2N4O10·3H2O, purity 94.9%), and reserpine (C33H40N2O9, purity > 99.9%) were purchased from national institutes for food and drug control (Beijing, China). Glyburide-d11 (C23H17ClD11N3O5S, purity 98%) was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Methanol (MeOH), acetonitrile (ACN), and methyl tert butyl ether (MTBE) were of HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA, USA). Acetic acid was obtained from Guangzhou chemical reagent factory (Guangzhou, China). Ammonium acetate was purchased from Macklin (Shanghai, China). Ultrapure water was freshly prepared using the Milli-Q Advantage A10 system (Milli-Q Reference, Millipore, Boston, MA, USA). Blank rat plasma samples were harvested from healthy adult rats supplied by the Laboratory Animal Center of Sun Yat-sen University (Guangdong, China).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!