The activity evaluation of the molecules was carried out in two different N. fowleri strains (ATCC® 30808™ and ATCC® 30215™) from the American Type Culture Collection (LG Promochem, Barcelona, Spain). The cytotoxicity of the compounds was evaluated using a murine macrophages cell line (ATCC® TIB-67). Cells were grown as previously described (Rizo-Liendo et al., 2020 (link)).
Atcc 30808
Manufactured by American Type Culture Collection
Sourced in Spain
The ATCC® 30808 is a laboratory incubator designed for cell and tissue culture applications. It maintains a controlled temperature and humidity environment suitable for the growth and maintenance of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Atcc 30215, by American Type Culture Collection (2 mentions)
Atcc tib 67, by American Type Culture Collection (1 mentions)
Penicillin g, by Merck Group (1 mentions)
Streptomycin sulphate, by Merck Group (1 mentions)
Penicillin g sodium salt, by Merck Group (1 mentions)
Bactocasitone medium, by Thermo Fisher Scientific (1 mentions)
Gentamicin, by Merck Group (1 mentions)
3 protocols using atcc 30808
1
Evaluation of Anti-Naegleria fowleri Activity
2
Amoeba Culture Protocols for Research
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The experiments were carried out using two amoeba species. N. fowleri type strain ATCC 30808™, from the American Type Culture Collection was used. The cells were axenically grown at 37°C in 2% bactocasitone medium supplemented with 10% of fetal bovine serum (FBS), 0.5 mg/mL of streptomycin sulphate and 0.3 μg/mL of penicillin G (Sigma Aldrich, Madrid, Spain). Clinical strain Acanthamoeba griffini genotype T3, obtained according to a previous study (González-Robles et al., 2014 (link)) was used. A. griffini trophozoites were grown axenically at 26°C in Peptone Yeast Glucose (PYG) medium (0.75% (w/v) proteose peptone, 0.75% (w/v) yeast extract, and 1.5% (w/v) glucose) containing 40 μg of gentamicin mL−1 (Biowest, Nuaillé, France).
3
Cultivation and Cytotoxicity of Naegleria fowleri
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Two type strains of Naegleria fowleri with reference number (ATCC® 30808™ and ATCC® 30215™) from the American Type Culture Collection (LG Promochem, Barcelona, Spain) were used in this study. Both strains were axenically cultured in 2% (w/v) Bactocasitone medium (Thermo Fisher Scientific, Madrid, Spain) at 37 °C, supplemented with 10% (v/v) of foetal bovine serum (FBS), containing 0,3 μg/mL of Penicillin G Sodium Salt and 0,5 mg/mL of Streptomycin sulphate (Sigma-Aldrich, Madrid, Spain). Amoebic strains were cultured in a biological security facility level 3 at the Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, regarding to the Spanish biosafety guidelines for this pathogen(Rizo-Liendo et al., 2019 (link)).
For the cytotoxicity assays, the murine macrophage J774A.1 (ATCC # TIB-67) cell line was used. The cells were cultured in Dulbecco's Modified Eagle's medium (DMEM, w/v) supplemented with 10% (v/v) FBS and 10 μg/mL gentamicin (Sigma-Aldrich, Madrid, Spain), at 37 °C in a 5% C02 atm (Sifaoui et al., 2017 (link)).
For the cytotoxicity assays, the murine macrophage J774A.1 (ATCC # TIB-67) cell line was used. The cells were cultured in Dulbecco's Modified Eagle's medium (DMEM, w/v) supplemented with 10% (v/v) FBS and 10 μg/mL gentamicin (Sigma-Aldrich, Madrid, Spain), at 37 °C in a 5% C02 atm (Sifaoui et al., 2017 (link)).
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