Glass 3881

Corning 96 well-black clear bottom plates (Corning Glass 3881, USA) were used to measure 80 μl of the liquid samples with 10 μM Thioflavin T (ThT) in triplicate (n = 3) under quiescent conditions. A 440 nm excitation filter and a 480 nm emission filter were used to record the difference in fluorescence (FLUOStar Galaxy from BMG Labtech, Offenburg, Germany). The ThT signal was neither saturated nor quenched as experiments with various ThT concentrations were performed without changes in the signal intensity. The absorbance at 360 nm was recorded to measure turbidity. For seeding experiments, a 100 mg/ml gel was formed at 37 °C, resuspended and then used for seeding at 5, 10, and 20% molar ratio. Data was analyzed with Prism 9.

The fluorescence intensity of His-NT-GFP (300 mg/ml, 80 μl) was measured in a Corning 96 well-black clear bottom plate (Corning Glass 3881, USA) in triplicate (n = 3) under quiescent conditions. The samples were measured with a fluorescence-based plate reader using an excitation wavelength of 395 nm and emission was recorded at 509 nm before gelation and after 2 h at 37 °C. Data were analyzed with Prism 9.